دورية أكاديمية
PNJ scaffolds promote microenvironmental regulation of glioblastoma stem-like cell enrichment and radioresistance
| العنوان: | PNJ scaffolds promote microenvironmental regulation of glioblastoma stem-like cell enrichment and radioresistance |
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| المؤلفون: | Heffernan, John M., McNamara, James B., Vernon, Brent L., Mehta, Shwetal, Sirianni, Rachael W. |
| المصدر: | Translational Neuroscience |
| بيانات النشر: | Barrow - St. Joseph's Scholarly Commons |
| سنة النشر: | 2022 |
| الوصف: | Glioblastoma (GBM) brain tumors contain a subpopulation of self-renewing multipotent Glioblastoma stem-like cells (GSCs) that are believed to drive the near inevitable recurrence of GBM. We previously engineered temperature responsive scaffolds based on the polymer poly(N-isopropylacrylamide-co-Jeffamine M-1000 acrylamide) (PNJ) for the purpose of enriching GSCs in vitro from patient-derived samples. Here, we used PNJ scaffolds to study microenvironmental regulation of self-renewal and radiation response in patient-derived GSCs representing classical and proneural subtypes. GSC self-renewal was regulated by the composition of PNJ scaffolds and varied with cell type. PNJ scaffolds protected against radiation-induced cell death, particularly in conditions that also promoted GSC self-renewal. Additionally, cells cultured in PNJ scaffolds exhibited increased expression of the transcription factor HIF2α, which was not observed in neurosphere culture, providing a potential mechanistic basis for differences in radio-resistance. Differences in PNJ regulation of HIF2α in irradiated and untreated conditions also offered evidence of stem plasticity. These data show PNJ scaffolds provide a unique biomaterial for evaluating dynamic microenvironmental regulation of GSC self-renewal, radioresistance, and stem plasticity. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://scholar.barrowneuro.org/neurobiology/1596Test; https://doi.org/10.1039/d0bm01169jTest |
| DOI: | 10.1039/d0bm01169j |
| الإتاحة: | https://doi.org/10.1039/d0bm01169jTest https://scholar.barrowneuro.org/neurobiology/1596Test |
| رقم الانضمام: | edsbas.DC727DB1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/d0bm01169j |
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