دورية أكاديمية
Changes of cellular stress response relatedhsp70andabcb1transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range
| العنوان: | Changes of cellular stress response relatedhsp70andabcb1transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range |
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| المؤلفون: | Protopopova, Marina V., Pavlichenko, Vasiliy V., Luckenbach, Till |
| المساهمون: | Deutscher Akademischer Austauschdienst, Russian Ministry of Education and Science, International DAAD scholarship, Erasmus Mundus (MULTIC II) scholarship, The Helmholtz Association, The Russian Foundation for Basic Research |
| المصدر: | PeerJ ; volume 8, page e8635 ; ISSN 2167-8359 |
| بيانات النشر: | PeerJ |
| سنة النشر: | 2020 |
| المجموعة: | PeerJ (E-Journal - via CrossRef) |
| الوصف: | The induction of cellular stress response systems, heat shock protein hsp70 /Hsp70 and multixenobiotic transporter abcb1 , by cadmium chloride (CdCl 2 ) was explored in amphipod species with different stress adaptation strategies from the Lake Baikal area. Based on the lethal concentrations (LC) of CdCl 2 , the sensitivities of the different species to CdCl 2 were ranked (24 hr LC50 in mg/L CdCl 2 (mean/95% confidence interval)): Gammarus lacustris (1.7/1.3–2.4) < Eulimnogammarus cyaneus (2.9/2.1–4.0) < Eulimnogammarus verrucosus (5.7/3.8–8.7) < Eulimnogammarus vittatus (18.1/12.4–26.6). Conjugated dienes, indicating lipid peroxidation, were significantly increased after 24 hr exposures to 5 mg/L CdCl 2 only in the more CdCl 2 -sensitive species G. lacustris and E. cyaneus . Upon treatment with 0.54 to 5.8 mg/L CdCl 2 for 1, 6 and 24 hrs, hsp70 transcript levels were generally more increased after the longer exposure times and in the more CdCl 2 -sensitive species. Relating the CdCl 2 exposure concentrations to LCx values revealed that across the species the increases of hsp70 transcript levels were comparatively low (up to 2.6-fold) at CdCl 2 concentrations ≤LC50. Relative hsp70 transcript levels were maximally increased in E. cyaneus by 5 mg/L CdCl 2 ($\hat {=}$LC70) at 24 hrs (9.1-fold increase above the respective control). When G. lacustris was exposed to 5 mg/L CdCl 2 ($\hat {=}$LC90) for 24 hrs, the increase in hsp70 was in comparison to E. cyaneus considerably less pronounced (3.0-fold increase in hsp70 levels relative to control). Upon exposure of amphipods to 5 mg/L CdCl 2 , increases in Hsp70 protein levels compared to untreated controls were highest in E. cyaneus at 1 and 6 hrs (5 mg/L CdCl 2 $\hat {=}$ LC70) and in E. verrucosus at 24 hrs (5 mg/L CdCl 2 $\hat {=}$ LC45). Thus, when the fold increases in Hsp70 protein levels in the different amphipod species were related to the respective species-specific LCx values a similar bell-shaped trend as for hsp70 transcript levels was seen across ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7717/peerj.8635 |
| الإتاحة: | https://doi.org/10.7717/peerj.8635Test https://peerj.com/articles/8635.pdfTest https://peerj.com/articles/8635.xmlTest https://peerj.com/articles/8635.htmlTest |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.DC3591F9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7717/peerj.8635 |
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