دورية أكاديمية
A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein
| العنوان: | A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein |
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| المؤلفون: | Kim, Cheolmin, Ryu, Dong-Kyun, Lee, Jihun, Kim, Young-Il, Seo, Ji-Min, Kim, Yeon-Gil, Jeong, Jae-Hee, Kim, Minsoo, Kim, Jong-In, Kim, Pankyeom, Bae, Jin Soo, Shim, Eun Yeong, Lee, Min Seob, Kim, Man Su, Noh, Hanmi, Park, Geun-Soo, Park, Jae Sang, Son, Dain, An, Yongjin, Lee, Jeong No, Kwon, Ki-Sung, Lee, Joo-Yeon, Lee, Hansaem, Yang, Jeong-Sun, Kim, Kyung-Chang, Kim, Sung Soon, Woo, Hye-Min, Kim, Jun-Won, Park, Man-Seong, Yu, Kwang-Min, Kim, Se-Mi, Kim, Eun-Ha, Park, Su-Jin, Jeong, Seong Tae, Yu, Chi Ho, Song, Youngjo, Gu, Se Hun, Oh, Hanseul, Koo, Bon-Sang, Hong, Jung Joo, Ryu, Choong-Min, Park, Wan Beom, Oh, Myoung-don, Choi, Young Ki, Lee, Soo-Young |
| المصدر: | Nature Communications ; volume 12, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary |
| الوصف: | Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting the receptor binding domain (RBD) of the viral spike protein via antibody library constructed from peripheral blood mononuclear cells of a convalescent patient. The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including the D614G variant without antibody-dependent enhancement effect. Complex crystal structure of CT-P59 Fab/RBD shows that CT-P59 blocks interaction regions of RBD for angiotensin converting enzyme 2 (ACE2) receptor with an orientation that is notably different from previously reported RBD-targeting mAbs. Furthermore, therapeutic effects of CT-P59 are evaluated in three animal models (ferret, hamster, and rhesus monkey), demonstrating a substantial reduction in viral titer along with alleviation of clinical symptoms. Therefore, CT-P59 may be a promising therapeutic candidate for COVID-19. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-020-20602-5 |
| الإتاحة: | https://doi.org/10.1038/s41467-020-20602-5Test https://www.nature.com/articles/s41467-020-20602-5.pdfTest https://www.nature.com/articles/s41467-020-20602-5Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.DC309A7C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-020-20602-5 |
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