دورية أكاديمية
Microdissected tissue vs tissue slices - a comparative study of tumor explant models cultured on-chip and off-chip
| العنوان: | Microdissected tissue vs tissue slices - a comparative study of tumor explant models cultured on-chip and off-chip |
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| المؤلفون: | Dorrigiv, Dina, Simeone, Kayla, Communal, Laudine, Kendall-Dupont, Jennifer, St-Georges-Robillard, Amélie, Péant, Benjamin, Carmona, Euridice, Mes-Masson, Anne-Marie, Gervais, Thomas |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | École Polytechnique de Montréal: PolyPublie |
| مصطلحات موضوعية: | 1900 Génie biomédical, 1901 Technologie biomédicale, 1902 Matériaux biomédicaux, 3100 Physique, 3101 Études atomiques et moléculaires |
| الوصف: | Predicting patient responses to anticancer drugs is a major challenge both at the drug development stage and during cancer treatment. Tumor explant culture platforms (TECPs) preserve the native tissue architecture and are well-suited for drug response assays. However, tissue longevity in these models is relatively low. Several methodologies have been developed to address this issue, although no study has compared their efficacy in a controlled fashion. We investigated the effect of two variables in TECPs, specifically, the tissue size and culture vessel on tissue survival using micro-dissected tumor tissue (MDT) and tissue slices which were cultured in microfluidic chips and plastic well plates. Tumor models were produced from ovarian and prostate cancer cell line xenografts and were matched in terms of the specimen, total volume of tissue, and respective volume of medium in each culture system. We examined morphology, viability, and hypoxia in the various tumor models. Our observations suggest that the viability and proliferative capacity of MDTs were not affected during the time course of the experiments. In contrast, tissue slices had reduced proliferation and showed increased cell death and hypoxia under both culture conditions. Tissue slices cultured in microfluidic devices had a lower degree of hypoxia compared to those in 96-well plates. Globally, our results show that tissue slices have lower survival rates compared to MDTs due to inherent diffusion limitations, and that microfluidic devices may decrease hypoxia in tumor models. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://publications.polymtl.ca/9372/1/2021_Dorrigiv_Microdissected_Tissue_vs_Tissue_Slices%E2%80%94A.pdfTest; Dorrigiv, D., Simeone, K., Communal, L., Kendall-Dupont, J., St-Georges-Robillard, A., Péant, B., Carmona, E., Mes-Masson, A.-M., & Gervais, T. (2021). Microdissected tissue vs tissue slices - a comparative study of tumor explant models cultured on-chip and off-chip. Cancers, 13(16), 4208 (14 pages). |
| DOI: | 10.3390/cancers13164208 |
| الإتاحة: | https://doi.org/10.3390/cancers13164208Test https://publications.polymtl.ca/9372Test/ https://publications.polymtl.ca/9372/1/2021_Dorrigiv_Microdissected_Tissue_vs_Tissue_Slices%E2%80%94A.pdfTest |
| حقوق: | cc_by |
| رقم الانضمام: | edsbas.DC01A0E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cancers13164208 |
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