دورية أكاديمية
Performance of the Cepheid Methicillin-Resistant Staphylococcus aureus/S. aureus Skin and Soft Tissue Infection PCR Assay on Respiratory Samples from Mechanically Ventilated Patients for S. aureus Screening during the Phase 2 Double-Blind SAATELLITE Study.
| العنوان: | Performance of the Cepheid Methicillin-Resistant Staphylococcus aureus/S. aureus Skin and Soft Tissue Infection PCR Assay on Respiratory Samples from Mechanically Ventilated Patients for S. aureus Screening during the Phase 2 Double-Blind SAATELLITE Study. |
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| المؤلفون: | Ruzin, Alexey, Barraud, Olivier, Yu, Li, François, Bruno, Sánchez-Garcia, Miguel, Eggimann, Philippe, Dequin, Pierre-François, Laterre, Pierre-François, Huberlant, Vincent, Viña, Lucia, Boulain, Thierry, Bretonniere, Cedric, Pugin, Jérôme, Trenado, José, Hernandez Padilla, Ana Catalina, Vignaud, Julie, Vandamme, Drieke, Goossens, Herman, Lammens, Christine, Ali, S Omar, Shoemaker, Kathryn, Ren, Pin, Colbert, Susan, Bellamy, Terramika, Sellman, Bret R, McCarthy, Michael, Jafri, Hasan S, Esser, Mark T, SAATELLITE Study Group |
| المساهمون: | UCL - SSS/IREC/MEDA - Pôle de médecine aiguë, UCL - (SLuc) Service de soins intensifs |
| المصدر: | Journal of clinical microbiology, Vol. 60, no.7, p. e0034722 [1-9] (2022) |
| بيانات النشر: | American Society for Microbiology. |
| سنة النشر: | 2022 |
| المجموعة: | DIAL@UCL (Université catholique de Louvain) |
| مصطلحات موضوعية: | Humans, Methicillin-Resistant Staphylococcus aureus, Real-Time Polymerase Chain Reaction, Respiration, Artificial, Soft Tissue Infections, Staphylococcal Infections, Staphylococcus aureus, SAATELLITE, qPCR, suvratoxumab, ventilator-associated pneumonia |
| الوصف: | We investigated the performance of the Xpert methicillin-resistant Staphylococcus aureus (MRSA)/S. aureus skin and soft tissue (SSTI) quantitative PCR (qPCR) assay in SAATELLITE, a multicenter, double-blind, phase 2 study of suvratoxumab, a monoclonal antibody (MAb) targeting S. aureus alpha-toxin, for reducing the incidence of S. aureus pneumonia. The assay was used to detect methicillin-susceptible S. aureus (MSSA) and MRSA in lower respiratory tract (LRT) samples from mechanically ventilated patients. LRT culture results were compared with S. aureus protein A () gene cycle threshold () values. Receiver operating characteristic (ROC) and Youden index were used to determine the cutoff for best separation of culture-S. aureus-negative and S. aureus-positive patients. Of 720 screened subjects, 299 (41.5%) were S. aureus positive by qPCR, of whom 209 had culture data: 162 (77.5%) were S. aureus positive and 47 (22.5%) were S. aureus negative. Culture results were negatively affected by antibiotic use and cross-laboratory variability. An inverse linear correlation was observed between values and quantitative S. aureus culture results. A value of 29 (≈2 × 10 CFU/mL) served as the best cutoff for separation between culture-negative and culture-positive samples. The associated area under the ROC curve was 83.8% (95% confidence interval [CI], 78 to 90%). Suvratoxumab provided greater reduction in S. aureus pneumonia or death than placebo in subjects with low S. aureus load ( ≥ 29; relative risk reduction [RRR], 50.0%; 90% CI, 2.7 to 74.4%) versus the total study population (RRR, 25.2%; 90% CI, -4.3 to 46.4%). The qPCR assay was easy to perform, sensitive, and standardized and provided better sensitivity than conventional culture for S. aureus detection. Quantitative PCR output correlated with suvratoxumab efficacy in reducing S. aureus pneumonia incidence or death in S. aureus-colonized, mechanically ventilated patients. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0095-1137 1098-660X |
| العلاقة: | boreal:279322; http://hdl.handle.net/2078.1/279322Test; info:pmid/35758652; urn:ISSN:0095-1137; urn:EISSN:1098-660X |
| DOI: | 10.1128/jcm.00347-22 |
| الإتاحة: | https://doi.org/10.1128/jcm.00347-22Test http://hdl.handle.net/2078.1/279322Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DA26CA6E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00951137 1098660X |
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| DOI: | 10.1128/jcm.00347-22 |