دورية أكاديمية
Recent advances for using human induced-pluripotent stem cells as pain-in-a-dish models of neuropathic pain
| العنوان: | Recent advances for using human induced-pluripotent stem cells as pain-in-a-dish models of neuropathic pain |
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| المؤلفون: | Labau, Julie I R, Andelic, Mirna, Faber, Catharina G, Waxman, Stephen G, Lauria, Giuseppe, Dib-Hajj, Sulayman |
| المساهمون: | J.I.R. Labau, M. Andelic, C.G. Faber, S.G. Waxman, G. Lauria, S. Dib-Hajj |
| سنة النشر: | 2022 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | 3D-sensory organoid, Human sensory neuron, Neuropathic pain, Patient-specific induced pluripotent stem cells, Settore MED/26 - Neurologia |
| الوصف: | Neuropathic pain is amongst the most common non-communicable disorders and the poor effectiveness of current treatment is an unmet need. Although pain is a universal experience, there are significant inter-individual phenotypic differences. Developing models that can accurately recapitulate the clinical pain features is crucial to better understand underlying pathophysiological mechanisms and find innovative treatments. Current data from heterologous expression systems that investigate properties of specific molecules involved in pain signaling, and from animal models, show limited success with their translation into the development of novel treatments for pain. This is in part because they do not recapitulate the native environment in which a particular molecule functions, and due to species-specific differences in the properties of several key molecules that are involved in pain signaling. The limited availability of post-mortem tissue, in particular dorsal root ganglia (DRG), has hampered research using human cells in pre-clinical studies. Human induced-pluripotent stem cells (iPSCs) have emerged as an exciting alternative platform to study patient-specific diseases. Sensory neurons that are derived from iPSCs (iPSC-SNs) have provided new avenues towards elucidating peripheral pathophysiological mechanisms, the potential for development of personalized treatments, and as a cell-based system for high-throughput screening for discovering novel analgesics. Nevertheless, reprogramming and differentiation protocols to obtain nociceptors have mostly yielded immature homogenous cell populations that do not recapitulate the heterogeneity of native sensory neurons. To close the gap between native human tissue and iPSCs, alternative strategies have been developed. We will review here recent developments in differentiating iPSC-SNs and their use in pre-clinical translational studies. Direct conversion of stem cells into the cells of interest has provided a more cost- and time-saving method to improve reproducibility and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/36100046; info:eu-repo/semantics/altIdentifier/wos/WOS:000867517900006; journal:EXPERIMENTAL NEUROLOGY; https://hdl.handle.net/2434/938048Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85138023151 |
| DOI: | 10.1016/j.expneurol.2022.114223 |
| الإتاحة: | https://doi.org/10.1016/j.expneurol.2022.114223Test https://hdl.handle.net/2434/938048Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DA1EEBBD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.expneurol.2022.114223 |
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