دورية أكاديمية
Intranasal delivery of the cytoplasmic domain of CTLA-4 using a novel protein transduction domain prevents allergic inflammation
| العنوان: | Intranasal delivery of the cytoplasmic domain of CTLA-4 using a novel protein transduction domain prevents allergic inflammation |
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| المساهمون: | Je-Min Choi, Mi-Hyun Ahn, Wook-Jin Chae, Yung-Gook Jung, Jae-Chul Park, Hyun-Mi Song, Young-Eun Kim, Jung-Ah Shin, Choon-Sik Park, Jung-Won Park, Tae-Kwann Park, Jung-Hoon Lee, Byung-Fhy Seo, Kyun-Do Kim, Eun-Sung Kim, Dong-Ho Lee, Seung-Kyou Lee, Sang-Kyou Lee, Park, Jung Won |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Administration, Intranasal, Animals, Antigens, CD, Differentiation/administration & dosage, Differentiation/genetics, Differentiation/immunology, Asthma*/immunology, Asthma*/prevention & control, Bronchial Hyperreactivity, CTLA-4 Antigen, Carrier Proteins/genetics, Carrier Proteins/metabolism, Female, Humans, Immunoconjugates/administration & dosage, Immunoconjugates/genetics, Immunoconjugates/immunology, Immunosuppressive Agents*/administration & dosage, Immunosuppressive Agents*/immunology, Inflammation*/immunology, Inflammation*/prevention & control, Male, Mice, Inbred BALB C, Ovalbumin/immunology, Polycomb Repressive Complex 1, Protein Structure, Tertiary |
| الوصف: | CTLA-4 is a negative regulator of T-cell activation, and its inhibitory effects can be accomplished either by competition with CD28 or by transmitting negative signals through its intracellular domain. To utilize the cytoplasmic domain of CTLA-4 to suppress allergic inflammation, we fused it to a novel protein-transduction domain in the human transcriptional factor Hph-1. Transduction efficiency was verified in vitro and in vivo after ocular, intranasal and intradermal administration. After transduction into T cells, the Hph-1-ctCTLA-4 fusion protein inhibited the production of interleukin (IL)-2, and downregulated CD69 and CD25. Intranasal administration of Hph-1-ctCTLA-4 resulted in markedly reduced infiltration of inflammatory cells, secretion of T helper type 2 (T(H)2) cytokines, serum IgE levels and airway hyper-responsiveness in a mouse model of allergic airway inflammation. These results indicated that Hph-1-ctCTLA-4 constitutes an effective immunosuppressive protein drug for potential use in the treatment of allergic asthma, via nasal administration. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 574~579 |
| اللغة: | unknown |
| تدمد: | 1078-8956 1546-170X |
| العلاقة: | NATURE MEDICINE; J02296; OAK-2006-00928; https://ir.ymlib.yonsei.ac.kr/handle/22282913/109668Test; http://www.nature.com/nm/journal/v12/n5/full/nm1385.htmlTest; T200600752; NATURE MEDICINE, Vol.12(5) : 574-579, 2006 |
| DOI: | 10.1038/nm1385 |
| الإتاحة: | https://doi.org/10.1038/nm1385Test https://ir.ymlib.yonsei.ac.kr/handle/22282913/109668Test http://www.nature.com/nm/journal/v12/n5/full/nm1385.htmlTest |
| حقوق: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/krTest/ ; not free |
| رقم الانضمام: | edsbas.D99131A4 |
| قاعدة البيانات: | BASE |
| تدمد: | 10788956 1546170X |
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| DOI: | 10.1038/nm1385 |