دورية أكاديمية
Prevention of Breast Cancer by Targeted Disruption of Breast Epithelial Cells
| العنوان: | Prevention of Breast Cancer by Targeted Disruption of Breast Epithelial Cells |
|---|---|
| المؤلفون: | Sukumar, Saraswati V. |
| المساهمون: | JOHNS HOPKINS UNIV BALTIMORE MD SCHOOLOF MEDICINE |
| المصدر: | DTIC AND NTIS |
| سنة النشر: | 2002 |
| المجموعة: | Defense Technical Information Center: DTIC Technical Reports database |
| مصطلحات موضوعية: | Anatomy and Physiology, Medicine and Medical Research, CELLS(BIOLOGY), BREAST CANCER, EPITHELIUM, KILL PROBABILITIES, VALIDATION, RATS, HUMANS, BACTERIA, PROTEINS, NEOPLASMS, TARGETS, PURIFICATION, LIGANDS, THERAPY, GENES, TRANSDUCERS, HYPOTHESES, RODENTS, PREVENTION, TOXINS AND ANTITOXINS, RECEPTOR SITES(PHYSIOLOGY), MAMMARY GLANDS, EPITHELIAL CELLS |
| الوصف: | We proposed to test the validity of the hypothesis that introduction of recombinant toxins into the confines of the mammary ductal tree through the teat will kill breast epithelial cells. The toxins TUF- and Heregulin-linked Pseudomonas exotoxin would be tested in the rat MNU-induced mammary tumor model. In the first year, we injected varying amounts of the TOF. a/PE and the Heregulin/PE toxin in rats by the intraductal route. While the toxin was extremely potent in human normal and breast cancer cells in culture, it was ineffective in killing the rat ductal cells. Although highly conserved, the human ligands do not appear to bind to the rodent receptors. We needed to design new toxins to target rat cells. We have completed the construction of a chimeric toxin consisting of the protein transduction domain of the HIV TAT gene to target and enter the cells, and the VPR gene of HIV to cause apoptosis. Expression of this protein in bacteria, and its purification is in progress. We have also demonstrated the efficacy of the intraductal route to cancer prevention and therapy using the NMU mammary tumor model. ; --Original contains color plates: All DTIC reproductions will be in black and white. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://www.dtic.mil/docs/citations/ADA408748Test |
| الإتاحة: | http://www.dtic.mil/docs/citations/ADA408748Test http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA408748Test |
| حقوق: | APPROVED FOR PUBLIC RELEASE |
| رقم الانضمام: | edsbas.D9708219 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |