التفاصيل البيبلوغرافية
| العنوان: |
Syt2 and Syt1 are differentially expressed at the mouse diaphragm and bladder. |
| المؤلفون: |
Hatim Thaker (7928519), Jie Zhang (64655), Shin-Ichiro Miyashita (6184568), Vivian Cristofaro (586878), SunHyun Park (11576924), Ali Hashemi Gheinani (11619876), Maryrose P. Sullivan (11576930), Rosalyn M. Adam (11576933), Min Dong (123378) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Neuroscience, Physiology, Pharmacology, Biotechnology, Evolutionary Biology, Immunology, Cancer, Infectious Diseases, Chemical Sciences not elsewhere classified, wild type mice, target neurons determines, skeletal neuromuscular junctions, potent toxins known, dominant toxin receptor, bonts &# 8217, analyzing bont action, dominant receptor, injecting toxins, wide range, two sets, therapeutic efficacy, overactive bladder, leg muscle, findings establish |
| الوصف: |
A. Immunohistochemistry analysis detected expression of Syt2, but not Syt1, in motor nerve terminals at WT mouse diaphragm neuromuscular junctions (NMJs). SMI-312 antibody detects neurofilament and marks the phrenic nerve. α-Bungarotoxin (a-btx) labels post-synaptic acetylcholine receptors and serves as a marker for NMJs. Scale bar, 20 μm. B. Immunohistochemistry analysis of bladder sections from WT mice detected expression of Syt1, but not Syt2 in neurons. β-3 tubulin marks nerve fibers and synapsin marks the pre-synaptic site of neuronal varicosities in bladder tissues. Scale bar, 20 μm. C. Diaphragms isolated from WT, Syt1 M3 KI, or Syt2 M3 KI mice were incubated with BoNT/B (100 nM, 90 min) in high K + buffer. Tissues were washed, and immunohistochemistry analysis was performed on whole mount tissues. BoNT/B binding and entry was detected on both WT and Syt1 M3 , but not Syt2 M3 diaphragms. D. Bladder tissues isolated from WT, Syt1 M3 , or Syt2 M3 mice were incubated with BoNT/B (100 nM, 90 min) in high K + buffer. Tissues were washed, and immunohistochemistry analysis was performed. BoNT/B binding and entry was detected on both WT and Syt2 M3 , but not Syt1 M3 bladders. Representative images were selected from n = 3–4 biological replicates. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/figure/Syt2_and_Syt1_are_differentially_expressed_at_the_mouse_diaphragm_and_bladder_/16827710Test |
| DOI: |
10.1371/journal.ppat.1009994.g002 |
| الإتاحة: |
https://doi.org/10.1371/journal.ppat.1009994.g002Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.D8D0790C |
| قاعدة البيانات: |
BASE |
