دورية أكاديمية
Listeria monocytogenes sensitivity to antimicrobial treatments depends on cell origin
| العنوان: | Listeria monocytogenes sensitivity to antimicrobial treatments depends on cell origin |
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| المؤلفون: | Montanari Chiara, Tabanelli Giulia, Barbieri Federica, Mora Diego, Duncan Robin, Gardini Fausto, Arioli Stefania |
| المساهمون: | Montanari Chiara, Tabanelli Giulia, Barbieri Federica, Mora Diego, Duncan Robin, Gardini Fausto, Arioli Stefania |
| سنة النشر: | 2021 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | Anti-Bacterial Agent, Bacterial Physiological Phenomena, Colony Count, Microbial, Culture Media, Disinfection, Food Microbiology, Listeria monocytogene, Listeriosi, Microbial Sensitivity Test, Microbial Viability, Temperature |
| الوصف: | In this study we investigated how cell origin could affect the efficacy of an antimicrobial treatment (mild heating combined with terpenoids) in Listeria monocytogenes Scott A, considering cells from: 1. single colony, 2. glycerol stock, 3. cold adapted culture, and 4. fresh culture in stationary phase. After treatment, culturability on BHI medium and viability assessed by flow cytometry were evaluated. Our results showed that the cell origin significantly impacted viability and culturability of L. monocytogenes towards antimicrobial treatment. The mild heat treatment combined or not with terpenoids mainly affected culturability rather than viability, although the culturability of cells from single colony was less impacted. Therefore, to mimic the worst scenario, these latter were selected to contaminate Gorgonzola rind and roast beef slices and we evaluated the ability of L. monocytogenes cells to recover their culturability (on ALOA agar medium) and to growth on the food matrix stored at 4°C for 7days. Our results suggest that only Gorgonzola rind allowed a partial recovery of the culturability of cells previously heated in presence or not of terpens. In conclusion, we found a connection between the cell history and sensitivity toward an antimicrobial treatment, underlying the importance to standardize the experimental procedures (starting from the cells to be used in the assay) in the assessment of cell sensitivity to a specific treatment. Finally, our study clearly indicated that VBNC cells can resuscitate under favorable conditions on a food matrix, becoming a threat for consumer’s health. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34711898; info:eu-repo/semantics/altIdentifier/wos/WOS:000712564800013; volume:11; issue:1; firstpage:1; lastpage:10; numberofpages:10; journal:SCIENTIFIC REPORTS; http://hdl.handle.net/11585/856315Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85118321745; https://www.nature.com/articles/s41598-021-00767-9Test |
| DOI: | 10.1038/s41598-021-00767-9 |
| الإتاحة: | https://doi.org/10.1038/s41598-021-00767-9Test http://hdl.handle.net/11585/856315Test https://www.nature.com/articles/s41598-021-00767-9Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.D7E885FA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-021-00767-9 |
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