دورية أكاديمية
Transforming growth factor-beta 1 produced by hippocampal cells modulates microglial reactivity in culture
| العنوان: | Transforming growth factor-beta 1 produced by hippocampal cells modulates microglial reactivity in culture |
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| المؤلفون: | Herrera Molina, R, von Bernhardi, R |
| بيانات النشر: | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| سنة النشر: | 2024 |
| المجموعة: | Pontificia Universidad Católica de Chile: Repositorio UC |
| مصطلحات موضوعية: | cytokines, hippocampal cell, inflammation, microglial cells, neurodegeneration, nitric oxide, superoxide anion, TGF beta 1, NITRIC-OXIDE SYNTHASE, INFLAMMATORY RESPONSE, TGF-BETA, OXIDATIVE STRESS, BRAIN-INJURY, IFN-GAMMA, NEURONS, ACTIVATION, EXPRESSION, TGF-BETA-1, 03 Good Health and Well-being, 03 Salud y bienestar |
| الوصف: | Activated microglia produce superoxide anion (O-2(center dot-)) and nitric oxide (NO), both of which can be neurotoxic. To identify regulatory mechanisms that might modulate over-activation of microglia, we evaluated the inhibition of microglial activation by factors secreted by hippocampal cells. Supernatants from hippocampal cell cultures (Hippocampal-Cm) prevented microglial O-2(center dot) and NO production. LAP-TGF beta 1 was present in the Hippocampal-Cm as shown by immunoblot and a TGF beta 1-dependent proliferation-inhibition bioassay. LAP-TGF beta 1 and TGF beta activity increased in hippocampal cultures exposed to proinflammatory conditions (LPS and Interferon-gamma). The inhibition of (O-2(center dot-)) and NO production by Hippocampal-Cm was mimicked by the addition of recombinant TGF beta 1. Treating Hippocampal-Cm with an antibody against TGF beta 1 to neutralize its activity eliminated its ability to inhibit O-2(center dot-) and NO production. Our findings suggest that the TGF beta 1 secreted by hippocampal cells modulated microglial activity. We propose that in pathological conditions, impairment of this modulatory mechanism could enhance microglia-mediated neurotoxicity. (c) 2005 Elsevier Inc. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 8 páginas; application/pdf |
| اللغة: | English |
| تدمد: | 1095-953X 0969-9961 |
| العلاقة: | MEDLINE:15837578; https://doi.org/10.1016/j.nbd.2005.01.003Test; https://repositorio.uc.cl/handle/11534/78819Test; WOS:000228672900024 |
| DOI: | 10.1016/j.nbd.2005.01.003 |
| الإتاحة: | https://doi.org/10.1016/j.nbd.2005.01.003Test https://repositorio.uc.cl/handle/11534/78819Test |
| حقوق: | acceso restringido |
| رقم الانضمام: | edsbas.D6AA2F94 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1095953X 09699961 |
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| DOI: | 10.1016/j.nbd.2005.01.003 |