دورية أكاديمية
Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex
| العنوان: | Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex |
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| المؤلفون: | Torrado, Mario, Low, Jason K. K., Silva, Ana P. G., Schmidberger, Jason W., Sana, Maryam, Sharifi Tabar, Mehdi, Isilak, Musa E., Winning, Courtney S., Kwong, Cherry, Bedward, Max J., Sperlazza, Mary J., Williams, David C., Shepherd, Nicholas E., Mackay, Joel P. |
| بيانات النشر: | Wiley-Blackwell Publishing |
| سنة النشر: | 2017 |
| المجموعة: | The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: | chromatin remodelling, co-immunoprecipitations, nucleosome remodelling and deacetylase (NuRD) complex, protein structure, protein-protein interactions, 1303 Biochemistry, 1307 Cell Biology, 1312 Molecular Biology |
| الوصف: | The nucleosome remodelling and deacetylase (NuRD) complex is essential for the development of complex animals. NuRD has roles in regulating gene expression and repairing damaged DNA. The complex comprises at least six proteins with two or more paralogues of each protein routinely identified when the complex is purified from cell extracts. To understand the structure and function of NuRD, a map of direct subunit interactions is needed. Dozens of published studies have attempted to define direct inter-subunit connectivities. We propose that conclusions reported in many such studies are in fact ambiguous for one of several reasons. First, the expression of many NuRD subunits in bacteria is unlikely to lead to folded, active protein. Second, interaction studies carried out in cells that contain endogenous NuRD complex can lead to false positives through bridging of target proteins by endogenous components. Combining existing information on NuRD structure with a protocol designed to minimize false positives, we report a conservative and robust interaction map for the NuRD complex. We also suggest a 3D model of the complex that brings together the existing data on the complex. The issues and strategies discussed herein are also applicable to the analysis of a wide range of multi-subunit complexes.Micrococcal nuclease (MNase), EC 3.1.31.1; histone deacetylase (HDAC), EC 3.5.1.98. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1742-4658 1742-464X |
| العلاقة: | orcid:0000-0001-6234-4456; APP1012161; 1063301; 571099; DE120102857; R01-GM098264; R01GM098264 |
| الإتاحة: | https://doi.org/10.1111/febs.14301Test https://espace.library.uq.edu.au/view/UQ:696815Test |
| رقم الانضمام: | edsbas.D6A365C6 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17424658 1742464X |
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