دورية أكاديمية
Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of normal human CD4+ T-lymphocytes
| العنوان: | Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of normal human CD4+ T-lymphocytes |
|---|---|
| المؤلفون: | Zhdanov D.D., Plyasova A.A., Pokrovsky V.S., Pokrovskaya M.V., Alexandrova S.S., Gladilina Y.A., Sokolov N.N. |
| المصدر: | Biochimie |
| بيانات النشر: | Elsevier B.V. |
| المجموعة: | RUDN University Open Repository (RUOR) |
| مصطلحات موضوعية: | Alternative splicing, Apoptosis progression, Deoxyribonuclease I, Lymphocytes, Splice-switching oligonucleotide |
| الوصف: | The nuclease activity of deoxyribonuclease 1 (DNase I) is regulated by alternative splicing (AS) of its mRNA. The aim of this study was to define the ability of a splice-switching oligonucleotide (SSO) that base-paired with DNase I pre-mRNA to induce AS and inhibit nuclease activity in human T, B and NK lymphocytes. The SSO for DNase I could significantly downregulate the expression of full-length active DNase I and upregulate a truncated splice variant with a deleted exon 4. Such an induction of AS resulted in inhibition of nuclease activity and slowed apoptosis progression in anti-CD95/FAS stimulated lymphocytes. These results should facilitate further investigations of apoptosis regulation in lymphocytes and demonstrate that SSOs for DNase I are promising cytoprotective agents. © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM) |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.biochi.2020.04.009Test; https://repository.rudn.ru/records/article/record/64583Test/ |
| الإتاحة: | https://doi.org/10.1016/j.biochi.2020.04.009Test https://repository.rudn.ru/records/article/record/64583Test/ |
| رقم الانضمام: | edsbas.D670CD78 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |