دورية أكاديمية
Elucidation of apoptosis induced by serum deprivation in cultured conjunctival epithelial cells
| العنوان: | Elucidation of apoptosis induced by serum deprivation in cultured conjunctival epithelial cells |
|---|---|
| المؤلفون: | Higuchi, A, Shimmura, S, Takeuchi, T, Suematsu, M, Tsubota, K |
| بيانات النشر: | British Medical Journal Publishing Group |
| سنة النشر: | 2006 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Laboratory science - Scientific reports |
| الوصف: | Background/aims: The conjunctival epithelial cell line, CCL20.2 (CCL), requires the presence of 10% fetal calf serum (FCS) in the medium to survive. To elucidate the molecular mechanism underlying such cell death, including the death signal for these cells, the activities of several caspases in the CCL were measured, and the effects of caspase inhibitors and serum components on cell death were examined. Methods: CCL was grown in Medium 199 containing 10% FCS, and the medium was changed to Medium 199 with or without 10% FCS, or medium without 10% FCS but containing caspase inhibitors or serum components. After 24 hours’ incubation, the enzyme activities of caspases 1, 3, 8, and 9 in the culture supernatants were measured, and the effects of caspase inhibitors and serum components—for example, growth factors, lactoferrin, retinoic acid, were investigated. Results: DNA fragmentation was induced by serum deprivation, confirming that serum deprivation induces apoptosis in CCL. While the activities of caspases 3 and 8 were found to be increased, those of caspases 1 and 9 were not detected in the apoptotic cells. Z-VAD completely suppressed the caspase 3 activation, and specific inhibitors of caspases 1, 8, and 9 partially suppressed the activation. Serum deprivation induced a decrease in the cellular viability, which, however, partially recovered in the presence of caspase inhibitors, epidermal growth factor and retinoic acid. Conclusion: These results suggest that the apoptosis induced by serum deprivation involves caspases 1, 3, 8, and 9, and is suppressed by caspase inhibitors. EGF and retinoic acid have a key role in the maintenance of the ocular surface. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://bjo.bmj.com/cgi/content/short/90/6/760Test; http://dx.doi.org/10.1136/bjo.2005.088203Test |
| DOI: | 10.1136/bjo.2005.088203 |
| الإتاحة: | https://doi.org/10.1136/bjo.2005.088203Test http://bjo.bmj.com/cgi/content/short/90/6/760Test |
| حقوق: | Copyright (C) 2006, BMJ Publishing Group Ltd |
| رقم الانضمام: | edsbas.D4913D6A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/bjo.2005.088203 |
|---|