Fecal Microbiota Transplantation in Gestating Sows and Neonatal Offspring Alters Lifetime Intestinal Microbiota and Growth in Offspring
| العنوان: | Fecal Microbiota Transplantation in Gestating Sows and Neonatal Offspring Alters Lifetime Intestinal Microbiota and Growth in Offspring |
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| المؤلفون: | McCormack, Ursula M., Curião, Tânia, Wilkinson, Toby, Metzler-Zebeli, Barbara U., Reyer, Henry, Ryan, Tomas, Calderon-Diaz, Julia A., Crispie, Fiona, Cotter, Paul D., Creevey, Christopher J., Gardiner, Gillian E., Lawlor, Peadar G., Adams, Rachel I. |
| المصدر: | mSystems, 3(3):e00134-17 |
| سنة النشر: | 2018 |
| المجموعة: | LeibnizOpen (The Leibniz Association) |
| مصطلحات موضوعية: | intestinal microbiota, pigs, fecal microbiota transplantation, feed efficiency |
| الوصف: | Previous studies suggest a link between intestinal microbiota and porcine feed efficiency (FE). Therefore, we investigated whether fecal microbiota transplantation (FMT) in sows and/or neonatal offspring, using inocula derived from highly feed-efficient pigs, could improve offspring FE. Pregnant sows were assigned to control or FMT treatments and the subsequent offspring to control treatment, FMT once (at birth), or FMT four times (between birth and weaning). FMT altered sow fecal and colostrum microbiota compositions and resulted in lighter offspring body weight at 70 and 155 days of age when administered to sows and/or offspring. This was accompanied by FMT-associated changes within the offspring’s intestinal microbiota, mostly in the ileum. These included transiently higher fecal bacterial diversity and load and numerous compositional differences at the phylum and genus levels (e.g., Spirochaetes and Bacteroidetes at high relative abundances and mostly members of Clostridia, respectively), as well as differences in the abundances of predicted bacterial pathways. In addition, intestinal morphology was negatively impacted, duodenal gene expression altered, and serum protein and cholesterol concentrations reduced due to FMT in sows and/or offspring. Taken together, the results suggest poorer absorptive capacity and intestinal health, most likely explaining the reduced body weight. An additive effect of FMT in sows and offspring also occurred for some parameters. Although these findings have negative implications for the practical use of the FMT regime used here for improving FE in pigs, they nonetheless demonstrate the enormous impact of early-life intestinal microbiota on the host phenotype. IMPORTANCE: Here, for the first time, we investigate FMT as a novel strategy to modulate the porcine intestinal microbiota in an attempt to improve FE in pigs. However, reprogramming the maternal and/or offspring microbiome by using fecal transplants derived from highly feed-efficient pigs did not recapitulate the highly ... |
| نوع الوثيقة: | other/unknown material |
| اللغة: | English |
| DOI: | 10.1128/mSystems.00134-17 |
| الإتاحة: | https://doi.org/10.1128/mSystems.00134-17Test https://repository.publisso.de/resource/frl:6410826Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864416Test/ https://msystems.asm.org/content/3/3/e00134-17/figures-onlyTest |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.D394D8D0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/mSystems.00134-17 |
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