دورية أكاديمية
FACS is more potent to fish IVD progenitor cells than magnetic and bead-based methods.
| العنوان: | FACS is more potent to fish IVD progenitor cells than magnetic and bead-based methods. |
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| المؤلفون: | Frauchiger, Daniela A, Tekari, Adel, May, Rahel Deborah, Džafo, Emina, Chan, Samantha Cw, Stoyanov, Jivko, Bertolo, Alessandro, Zhang, Xingshuo, Guerrero, Julien, Sakai, Daisuke, Schol, Jordy, Grad, Sibylle, Tryfonidou, Marianna A, Benneker, Lorin M., Gantenbein, Benjamin |
| المصدر: | Frauchiger, Daniela A; Tekari, Adel; May, Rahel Deborah; Džafo, Emina; Chan, Samantha Cw; Stoyanov, Jivko; Bertolo, Alessandro; Zhang, Xingshuo; Guerrero, Julien; Sakai, Daisuke; Schol, Jordy; Grad, Sibylle; Tryfonidou, Marianna A; Benneker, Lorin M.; Gantenbein, Benjamin (2019). FACS is more potent to fish IVD progenitor cells than magnetic and bead-based methods. Tissue engineering. Part C, Methods, 25(10), pp. 571-580. Mary Ann Liebert 10.1089/ten.TEC.2018.0375 |
| بيانات النشر: | Mary Ann Liebert |
| سنة النشر: | 2019 |
| المجموعة: | BORIS (Bern Open Repository and Information System, University of Bern) |
| مصطلحات موضوعية: | 610 Medicine & health, 570 Life sciences, biology |
| الوصف: | Low back pain related to intervertebral disc (IVD) degeneration has a major socioeconomical impact on our ageing society. Therefore, stem cell therapy to activate self-repair of the IVD remains an exciting treatment strategy. In this respect, tissue-specific progenitors may play a crucial role for IVD regeneration, as these cells are perfectly adapted to this niche. Such a rare progenitor cell population residing in the nucleus pulposus (nucleus pulposus progenitor cells or NPPCs) was found positive for the angiopoietin-1 receptor (Tie2+) and was demonstrated to possess self-renewal capacity and in vitro multipotency. Here, we compared three sorting protocols, i.e., fluorescence-activated cell sorting (FACS), magnetic-activated cell sorting (MACS) and a mesh-based label-free cell sorting system (pluriSelect), with respect to cell yield, potential to form colonies (colony forming units = CFUs) and in vitro functional differentiation assays for tripotency. The aim of this study was to demonstrate efficiency of three wide-spread cell sorting methods for picking rare cells (<5%) and how these isolated cells then behave in down-stream functional differentiation in adipogenesis, osteogenesis and chondrogenesis. The cell yield among the isolation methods differed widely, with FACS presenting the highest yield (5.0 ± 4.0%), followed by MACS (1.6 ± 2.9%) and pluriSelect (1.1 ± 1.0%). The number of colonies formed was not significantly different between Tie2+ and Tie2- NPPCs. Only FACS was able to separate into two functionally different populations that showed trilineage multipotency, while MACS and pluriSelect failed to maintain a clear separation between Tie2+ and Tie2- populations in differentiation assays. To conclude, the isolation of NPPC was possible with all three sorting methods, while FACS was the preferred technique for separation of functional Tie2+ cells. . |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://boris.unibe.ch/132936Test/ |
| الإتاحة: | https://doi.org/10.1089/ten.TEC.2018.0375Test https://boris.unibe.ch/132936/1/TEC-2018-0375-Frauchiger_authorcopy.pdfTest https://boris.unibe.ch/132936Test/ |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.CDE0ED9E |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |