التفاصيل البيبلوغرافية
| العنوان: |
miR-146a and miR-370 coordinate enterovirus 71-induced cell apoptosis through targeting SOS1 and GADD45 |
| المؤلفون: |
Chang, Ya-Ling, Ho, Bing-Ching, Sher, Singh, Yu, Sung-Liang, Yang, Pan-Chyr, 何炳慶, 俞松良, 楊泮池 |
| المساهمون: |
臺大醫院-內科部, 臺大醫院-檢驗醫學部, 臺大醫學院, 臺大醫學院-分子醫學研究所, 臺大醫學院-光電生物醫學研究中心, 臺大醫學院-病理學科暨研究所, 臺大醫學院-基因體暨蛋白體醫學研究所, 臺大醫學院-醫學檢驗暨生物技術學系暨研究所, Chang, Ya-Ling, Ho, Bing-Ching, Sher, Singh, Yu, Sung-Liang, Yang, Pan-Chyr, 何炳慶, 俞松良, 楊泮池 |
| سنة النشر: |
2015 |
| المجموعة: |
National Taiwan University Institutional Repository (NTUR) |
| الوقت: |
136 |
| الوصف: |
Enterovirus 71 (EV71) is an emerging life-threatening pathogen particularly in the Asia-Pacific region. The major pathogenic feature in EV71-infected cells is apoptosis. However, which molecular mechanism mainly contributes to EV71-induced apoptosis is not investigated thoroughly. MicroRNAs (MiRNAs), the newly discovered molecules, govern a wide range of biological functions through post-transcriptional regulation including viral pathogenesis. Whether miRNAs and messenger RNAs (mRNAs) coordinate to trigger host cell apoptosis in EV71 infection was investigated in this study. We conducted an apoptosis-oriented approach using both mRNA and miRNA profiling and bioinformatic analysis. We identified two major apoptosis-associated signalling pathways, Bcl2 antagonist of cell death (BAD) phosphorylation and p53-dependent apoptosis pathways, in which Son of sevenless homolog 1 (SOS1) and Growth arrest and DNA damage-inducible protein 45 (GADD45) were predicted as targets of miR-146a and miR-370 respectively. Luciferase reporter assays and Western blots demonstrated the negative regulation between miR-146a and SOS1 and between miR-370 and GADD45. Silencing of miR-146a restored SOS1 expression and partially attenuated EV71 infection-induced apoptosis. Conversely, ectopic expression of miR-370 decreased virus infection-induced GADD45 expression and also diminished apoptosis. Finally, the transfection of antagomiR-146a and miR-370 contributed to attenuating EV71 infection-induced apoptosis. Herein we clearly demonstrate that EV71-induced cell apoptosis is partly governed by altered miRNAs. ; 病理學科暨研究所 ; 醫學院 ; 期刊論文 |
| نوع الوثيقة: |
other/unknown material |
| اللغة: |
English |
| العلاقة: |
Cell Microbiol., 17(6), 802-818; http://ntur.lib.ntu.edu.tw/handle/246246/278634Test |
| DOI: |
10.1111/cmi.12401 |
| الإتاحة: |
https://doi.org/10.1111/cmi.12401Test
http://ntur.lib.ntu.edu.tw/handle/246246/278634Test |
| رقم الانضمام: |
edsbas.CDA56A96 |
| قاعدة البيانات: |
BASE |
