دورية أكاديمية
Low unspliced cell-associated HIV RNA in early treated adolescents living with HIV on long suppressive ART
| العنوان: | Low unspliced cell-associated HIV RNA in early treated adolescents living with HIV on long suppressive ART |
|---|---|
| المؤلفون: | Gärtner, K, Domínguez-Rodríguez, S, Heaney, J, Gkouleli, T, Grant, P, Dorgham, K, Sauce, D, Soulie, C, Busby, EJ, O’Sullivan, DM, Spyer, M, Botha, JC, Muñoz-Fernandez, MA, Tagarro, A, Cotugno, N, Huggett, JF, Klein, N, Palma, P, Rojo Conejo, P, Foster, C, Giaquinto, C, Rossi, P, Persaud, D, De Rossi, A, Marcelin, AG, Nastouli, E, EPIICAL consortium |
| المصدر: | Frontiers in Immunology , 15 , Article 1334236. (2024) |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2024 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | HIV-1, adolescents, early treated, suppressed, cell-associated RNA, cell-associated DNA, cytokines |
| الوصف: | Introduction: Initiation of antiretroviral treatment (ART) in patients early after HIV-infection and long-term suppression leads to low or undetectable levels of HIV RNA and cell-associated (CA) HIV DNA and RNA. Both CA-DNA and CA-RNA, overestimate the size of the HIV reservoir but CA-RNA as well as p24/cell-free viral RNA can be indicators of residual viral replication. This study describes HIV RNA amounts and levels of cytokines/soluble markers in 40 well-suppressed adolescents who initiated ART early in life and investigated which viral markers may be informative as endpoints in cure clinical trials within this population. // Methods: Forty adolescents perinatally infected with HIV on suppressive ART for >5 years were enrolled in the CARMA study. HIV DNA and total or unspliced CA-RNA in PBMCs were analyzed by qPCR/RT-qPCR and dPCR/RT-dPCR. Cell-free HIV was determined using an ultrasensitive viral load (US-VL) assay. Plasma markers and p24 were analyzed by digital ELISA and correlations between total and unspliced HIV RNA and clinical markers, including age at ART, Western Blot score, levels of cytokines/inflammation markers or HIV CA-DNA, were tested. // Results: CA-RNA was detected in two thirds of the participants and was comparable in RT-qPCR and RT-dPCR. Adolescents with undetectable CA-RNA showed significantly lower HIV DNA compared to individuals with detectable CA-RNA. Undetectable unspliced CA-RNA was positively associated with age at ART initiation and Western Blot score. We found that a higher concentration of TNF-α was predictive of higher CA-DNA and CA-RNA. Other clinical characteristics like US-VL, time to suppression, or percent CD4+ T-lymphocytes were not predictive of the CA-RNA in this cross-sectional study. // Conclusions: Low CA-DNA after long-term suppressive ART is associated with lower CA-RNA, in concordance with other reports. Patients with low CA-RNA levels in combination with low CA-DNA and low Western Blot scores should be further investigated to characterize candidates for ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://discovery.ucl.ac.uk/id/eprint/10189541/1/fimmu-15-1334236.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10189541Test/ |
| الإتاحة: | https://discovery.ucl.ac.uk/id/eprint/10189541/1/fimmu-15-1334236.pdfTest https://discovery.ucl.ac.uk/id/eprint/10189541Test/ |
| حقوق: | open |
| رقم الانضمام: | edsbas.CD59573C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |