التفاصيل البيبلوغرافية
| العنوان: |
Protein Markers Quality Control of Serum Albumin Depletion for Proteomic Analysis |
| المؤلفون: |
Nitin Seam, Denise A. Gonzales, Steven J. Kern, Glen L. Hortin, Gerard T. Hoehn, Anthony F. Suffredini |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
http://www.clinchem.org/content/early/2007/09/21/clinchem.2007.091736.full.pdfTest. |
| سنة النشر: |
2007 |
| المجموعة: |
CiteSeerX |
| الوصف: |
Background: Prefractionation techniques such as serum albumin depletion are useful precursors to proteomic analysis, but they may introduce preanalytical bias if the depletion is not reproducible. We examined the reproducibility of albumin immunodepletion and describe a method of quality control for this process. Methods: Depletion of albumin from pooled serum, performed using IgY immunoaffinity spin columns, was assessed for 21 runs on each of 4 columns. We measured albumin concentrations, after albumin depletion, by use of an immunoturbidimetric assay on the Beckman LX 20 analyzer and assessed mass spectra of albumin-depleted samples by use of SELDI-TOF mass spectrometry. Results: There was substantial run-to-run variation in efficiency of albumin depletion, with systematic decline in efficiency after multiple uses of the columns. Mean depletion efficiency was>95 % for 15 of 17 runs and <90 % for runs 18 to 21. We evaluated the 10 highestintensity peaks present in all spectra from runs 1, 8, 17, and 21 and assessed the effect of albumin depletion on SELDI-TOF mass spectrometry reproducibility. Comparing the %CV of relative intensities for low and high m/z measurements revealed a significant difference of run 21 compared with runs 1, 8, and 17 (P <0.0001). Six-fold more peaks were found in albumin-depleted than unfractionated serum at both high and low m/z. 1 |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.323.2064Test; http://www.clinchem.org/content/early/2007/09/21/clinchem.2007.091736.full.pdfTest |
| الإتاحة: |
http://www.clinchem.org/content/early/2007/09/21/clinchem.2007.091736.full.pdfTest |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.CBFE2796 |
| قاعدة البيانات: |
BASE |
