دورية أكاديمية
Global transcriptome profiling and functional analysis reveal that tissue-specific constitutive overexpression of cytochrome P450s confers tolerance to imidacloprid in palm weevils in date palm fields.
| العنوان: | Global transcriptome profiling and functional analysis reveal that tissue-specific constitutive overexpression of cytochrome P450s confers tolerance to imidacloprid in palm weevils in date palm fields. |
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| المؤلفون: | Antony, Binu, Johny, Jibin, Abdelazim, Mahmoud M, Jakše, Jernej, Al-Saleh, Mohammed Ali, Pain, Arnab |
| المساهمون: | Bioscience Program, Biological and Environmental Sciences and Engineering (BESE) Division, Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Chair of Date Palm Research, Riyadh, 11451, Saudi Arabia., Biotechnical Faculty, Agronomy Department, University of Ljubljana, SI-1000, Ljubljana, Slovenia. |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2019 |
| المجموعة: | King Abdullah University of Science and Technology: KAUST Repository |
| الوصف: | BACKGROUND:Cytochrome P450-dependent monooxygenases (P450s), constituting one of the largest and oldest gene superfamilies found in many organisms from bacteria to humans, play a vital role in the detoxification and inactivation of endogenous toxic compounds. The use of various insecticides has increased over the last two decades, and insects have developed resistance to most of these compounds through the detoxifying function of P450s. In this study, we focused on the red palm weevil (RPW), Rhynchophorus ferrugineus, the most devastating pest of palm trees worldwide, and demonstrated through functional analysis that upregulation of P450 gene expression has evolved as an adaptation to insecticide stress arising from exposure to the neonicotinoid-class systematic insecticide imidacloprid. RESULTS:Based on the RPW global transcriptome analysis, we identified 101 putative P450 genes, including 77 likely encoding protein coding genes with ubiquitous expression. A phylogenetic analysis revealed extensive functional and species-specific diversification of RPW P450s, indicating that multiple CYPs actively participated in the detoxification process. We identified highly conserved paralogs of insect P450s that likely play a role in the development of resistance to imidacloprid: Drosophila Cyp6g1 (CYP6345J1) and Bemisia tabaci CYP4C64 (CYP4LE1). We performed a toxicity bioassay and evaluated the induction of P450s, followed by the identification of overexpressed P450s, including CYP9Z82, CYP6fra5, CYP6NR1, CYP6345J1 and CYP4BD4, which confer cross-resistance to imidacloprid. In addition, under imidacloprid insecticide stress in a date palm field, we observed increased expression of various P450 genes, with CYP9Z82, CYP4BD4, CYP6NR1 and CYP6345J1 being the most upregulated detoxification genes in RPWs. Expression profiling and cluster analysis revealed P450 genes with multiple patterns of induction and differential expression. Furthermore, we used RNA interference to knock down the overexpressed P450s, after which a ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; image/tiff; application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
| اللغة: | unknown |
| العلاقة: | https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5837-4Test; Antony, B., Johny, J., Abdelazim, M. M., Jakše, J., Al-Saleh, M. A., & Pain, A. (2019). Global transcriptome profiling and functional analysis reveal that tissue-specific constitutive overexpression of cytochrome P450s confers tolerance to imidacloprid in palm weevils in date palm fields. BMC Genomics, 20(1). doi:10.1186/s12864-019-5837-4; BMC genomics; http://hdl.handle.net/10754/656354Test |
| DOI: | 10.1186/s12864-019-5837-4 |
| الإتاحة: | https://doi.org/10.1186/s12864-019-5837-4Test https://doi.org/10.6084/m9.figshare.c.4526078.v1Test http://hdl.handle.net/10754/656354Test |
| حقوق: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0Test/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0Test/) applies to the data made available in this article, unless otherwise stated. ; http://creativecommons.org/publicdomain/zero/1.0Test/ |
| رقم الانضمام: | edsbas.CBC3672A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12864-019-5837-4 |
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