دورية أكاديمية
The Listeria monocytogenes PASTA Kinase PrkA and Its Substrate YvcK Are Required for Cell Wall Homeostasis, Metabolism, and Virulence.
| العنوان: | The Listeria monocytogenes PASTA Kinase PrkA and Its Substrate YvcK Are Required for Cell Wall Homeostasis, Metabolism, and Virulence. |
|---|---|
| المؤلفون: | Daniel A Pensinger, Kyle M Boldon, Grischa Y Chen, William J B Vincent, Kyle Sherman, Meng Xiong, Adam J Schaenzer, Emily R Forster, Jörn Coers, Rob Striker, John-Demian Sauer |
| المصدر: | PLoS Pathogens, Vol 12, Iss 11, p e1006001 (2016) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2016 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5 |
| الوصف: | Obstacles to bacterial survival and replication in the cytosol of host cells, and the mechanisms used by bacterial pathogens to adapt to this niche are not well understood. Listeria monocytogenes is a well-studied Gram-positive foodborne pathogen that has evolved to invade and replicate within the host cell cytosol; yet the mechanisms by which it senses and responds to stress to survive in the cytosol are largely unknown. To assess the role of the L. monocytogenes penicillin-binding-protein and serine/threonine associated (PASTA) kinase PrkA in stress responses, cytosolic survival and virulence, we constructed a ΔprkA deletion mutant. PrkA was required for resistance to cell wall stress, growth on cytosolic carbon sources, intracellular replication, cytosolic survival, inflammasome avoidance and ultimately virulence in a murine model of Listeriosis. In Bacillus subtilis and Mycobacterium tuberculosis, homologues of PrkA phosphorylate a highly conserved protein of unknown function, YvcK. We found that, similar to PrkA, YvcK is also required for cell wall stress responses, metabolism of glycerol, cytosolic survival, inflammasome avoidance and virulence. We further demonstrate that similar to other organisms, YvcK is directly phosphorylated by PrkA, although the specific site(s) of phosphorylation are not highly conserved. Finally, analysis of phosphoablative and phosphomimetic mutants of YvcK in vitro and in vivo demonstrate that while phosphorylation of YvcK is irrelevant to metabolism and cell wall stress responses, surprisingly, a phosphomimetic, nonreversible negative charge of YvcK is detrimental to cytosolic survival and virulence in vivo. Taken together our data identify two novel virulence factors essential for cytosolic survival and virulence of L. monocytogenes. Furthermore, our data demonstrate that regulation of YvcK phosphorylation is tightly controlled and is critical for virulence. Finally, our data suggest that yet to be identified substrates of PrkA are essential for cytosolic survival and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1553-7366 1553-7374 |
| العلاقة: | https://doi.org/10.1371/journal.ppat.1006001Test; https://doaj.org/toc/1553-7366Test; https://doaj.org/toc/1553-7374Test; https://doaj.org/article/48519dc5c4b54cbcb6a2eb08370eb5aeTest |
| DOI: | 10.1371/journal.ppat.1006001 |
| الإتاحة: | https://doi.org/10.1371/journal.ppat.1006001Test https://doaj.org/article/48519dc5c4b54cbcb6a2eb08370eb5aeTest |
| رقم الانضمام: | edsbas.CB80258C |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 15537366 15537374 |
|---|---|
| DOI: | 10.1371/journal.ppat.1006001 |