صورة
Image_3_Extended Combined Neonatal Treatment With Erythropoietin Plus Melatonin Prevents Posthemorrhagic Hydrocephalus of Prematurity in Rats.JPEG
| العنوان: | Image_3_Extended Combined Neonatal Treatment With Erythropoietin Plus Melatonin Prevents Posthemorrhagic Hydrocephalus of Prematurity in Rats.JPEG |
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| المؤلفون: | Shenandoah Robinson, Fatu S. Conteh, Akosua Y. Oppong, Tracylyn R. Yellowhair, Jessie C. Newville, Nagat El Demerdash, Christine L. Shrock, Jessie R. Maxwell, Stephen Jett, Frances J. Northington, Lauren L. Jantzie |
| سنة النشر: | 2018 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Cell Biology, Neuroscience, Cellular Nervous System, Central Nervous System, Cellular Interactions (incl. Adhesion, Matrix, Cell Wall), Protein Trafficking, intraventricular hemorrhage (IVH), chorioamnionitis, neurorepair, ventriculomegaly, diffusion tensor imaging (DTI), preterm, cilia, cerebrospinal fluid |
| الوصف: | Posthemorrhagic hydrocephalus of prematurity (PHHP) remains a global challenge. Early preterm infants (<32 weeks gestation), particularly those exposed to chorioamnionitis (CAM), are prone to intraventricular hemorrhage (IVH) and PHHP. We established an age-appropriate, preclinical model of PHHP with progressive macrocephaly and ventriculomegaly to test whether non-surgical neonatal treatment could modulate PHHP. We combined prenatal CAM and postnatal day 1 (P1, equivalent to 30 weeks human gestation) IVH in rats, and administered systemic erythropoietin (EPO) plus melatonin (MLT), or vehicle, from P2 to P10. CAM-IVH rats developed progressive macrocephaly through P21. Macrocephaly was accompanied by ventriculomegaly at P5 (histology), and P21 (ex vivo MRI). CAM-IVH rats showed impaired performance of cliff aversion, a neonatal neurodevelopmental test. Neonatal EPO+MLT treatment prevented macrocephaly and cliff aversion impairment, and significantly reduced ventriculomegaly. EPO+MLT treatment prevented matted or missing ependymal motile cilia observed in vehicle-treated CAM-IVH rats. EPO+MLT treatment also normalized ependymal yes-associated protein (YAP) mRNA levels, and reduced ependymal GFAP-immunolabeling. Vehicle-treated CAM-IVH rats exhibited loss of microstructural integrity on diffusion tensor imaging, which was normalized in EPO+MLT-treated CAM-IVH rats. In summary, combined prenatal systemic inflammation plus early postnatal IVH caused progressive macrocephaly, ventriculomegaly and delayed development of cliff aversion reminiscent of PHHP. Neonatal systemic EPO+MLT treatment prevented multiple hallmarks of PHHP, consistent with a clinically viable, non-surgical treatment strategy. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/figure/Image_3_Extended_Combined_Neonatal_Treatment_With_Erythropoietin_Plus_Melatonin_Prevents_Posthemorrhagic_Hydrocephalus_of_Prematurity_in_Rats_JPEG/7126724Test |
| DOI: | 10.3389/fncel.2018.00322.s003 |
| الإتاحة: | https://doi.org/10.3389/fncel.2018.00322.s003Test https://figshare.com/articles/figure/Image_3_Extended_Combined_Neonatal_Treatment_With_Erythropoietin_Plus_Melatonin_Prevents_Posthemorrhagic_Hydrocephalus_of_Prematurity_in_Rats_JPEG/7126724Test |
| حقوق: | CC BY 4.0 |
| رقم الانضمام: | edsbas.CB62E5FE |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fncel.2018.00322.s003 |
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