تقرير
Unraveling human, rodent and snake Kolmioviridae replication to anticipate cross-species transmission
| العنوان: | Unraveling human, rodent and snake Kolmioviridae replication to anticipate cross-species transmission |
|---|---|
| المؤلفون: | Khalfi, Pierre, Denis, Zoé, Merolla, Giovanni, Chavey, Carine, Ursic-Bedoya, José, Soppa, Lena, Szirovicza, Leonora, Hetzel, Udo, Dufourt, Jeremy, Leyrat, Cedric, Goldmann, Nora, Goto, Kaku, Verrier, Eloi, Baumert, Thomas, F, Glebe, Dieter, Courgnaud, Valérie, Grégoire, Damien, Hepojoki, Jussi, Majzoub, Karim |
| المساهمون: | Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Universität Zürich Zürich = University of Zurich (UZH), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Institut de Recherche sur les Maladies Virales et Hépatiques (IVH), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Virology Helsinki, Haartman Institute Helsinki, Faculty of Medecine Helsinki, Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Faculty of Medecine Helsinki, Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Helsingin yliopisto = Helsingfors universitet = University of Helsinki, The project was funded by the ATIP-AVENIR program (CNRS- INSERM), the MUSE (Montpellier Université d’Excellence) grant, by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) to D.G. project number 197785619 (SFB 1021) and the European Research Council (ERC) starting grant 101039538 – DELV. |
| المصدر: | https://hal.science/hal-04209863Test ; 2023. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2023 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | HDV-like viruses, HDV, hydrodynamic injections, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | The recent discovery of Hepatitis D (HDV)-like viruses across a wide range of taxa led to the establishment of the Kolmioviridae family. Recent studies suggest that kolmiovirids can be satellites of viruses other than Hepatitis B virus (HBV), challenging the strict HBV/HDV-association dogma. Studying whether kolmiovirids are able to replicate in any animal cell they enter is essential to assess their zoonotic potential. Here, we compared replication of three kolmiovirids: HDV, rodent (RDeV) and snake deltavirus (SDeV) in vitro and in vivo. We show that SDeV has the narrowest and RDeV the broadest host cell range. High resolution imaging of infected cells revealed nuclear viral hubs with a peculiar RNA-protein organization. Finally, in vivo hydrodynamic delivery of infectious clones showed that both HDV and RDeV, but not SDeV, efficiently replicate in mouse liver, forming massive nuclear viral hubs. Our comparative analysis lays the foundation for the discovery of specific host factors controlling Kolmioviridae host-shifting. |
| نوع الوثيقة: | report |
| اللغة: | English |
| العلاقة: | hal-04209863; https://hal.science/hal-04209863Test; https://hal.science/hal-04209863/documentTest; https://hal.science/hal-04209863/file/2023%20Khalfi,%20Denis%20et%20al.%20biorxiv.pdfTest; BIORXIV: 2023.05.17.541162 |
| الإتاحة: | https://hal.science/hal-04209863Test https://hal.science/hal-04209863/documentTest https://hal.science/hal-04209863/file/2023%20Khalfi,%20Denis%20et%20al.%20biorxiv.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.CAB1F14A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |