دورية أكاديمية
A New Approach for Rapidly Reshaping Single-Chain Antibody In Vitro by Combining DNA Shuffling with Ribosome Display
| العنوان: | A New Approach for Rapidly Reshaping Single-Chain Antibody In Vitro by Combining DNA Shuffling with Ribosome Display |
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| المؤلفون: | Wang, Xiang-Bin, Zhou, Bing, Yin, Chang-Cheng, Lin, Qing, Huang, Hua-Liang |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2004 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | BIOTECHNOLOGY |
| الوصف: | Antibody reshaping is an effective way to reduce the immunogenicity while maintaining or improving the affinity of murine antibodies. This paper describe a new in vitro approach for rapidly reshaping murine antibodies by combining DNA shuffling with ribosome display. With the new method, a reshaping anti–4-1BB single-chain antibody (scFv), Re-4B4-1 scFv, which bound to its antigen (4-1BB) specifically and strongly, was selected from a reshaping library. These results proved definitely the feasibility of the new designed approach for antibody reshaping. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://jb.oxfordjournals.org/cgi/content/short/136/1/19Test; http://dx.doi.org/10.1093/jb/mvh083Test |
| DOI: | 10.1093/jb/mvh083 |
| الإتاحة: | https://doi.org/10.1093/jb/mvh083Test http://jb.oxfordjournals.org/cgi/content/short/136/1/19Test |
| حقوق: | Copyright (C) 2004, Japanese Biochemical Society |
| رقم الانضمام: | edsbas.CA8A6268 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jb/mvh083 |
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