التفاصيل البيبلوغرافية
| العنوان: |
Tumor cell and immune cell profiles in primary human glioblastoma: Impact on patient outcome |
| المؤلفون: |
González‐Tablas Pimenta, María, Otero, Álvaro, Arandia Guzman, Daniel Angel, Pascual‐Argente, Daniel, Ruíz Martín, Laura, Sousa‐Casasnovas, Pablo, García‐Martin, Andoni, Roa Montes de Oca, Juan Carlos, Villaseñor‐Ledezma, Javier, Torres Carretero, Luis, Almeida, Maria, Ortiz, Javie, Nieto, Adelaida, Orfao, Alberto, Tabernero, María Dolores |
| المصدر: |
Brain Pathology ; volume 31, issue 2, page 365-380 ; ISSN 1015-6305 1750-3639 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2021 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
The distribution and role of tumor‐infiltrating leucocytes in glioblastoma (GBM) remain largely unknown. Here, we investigated the cellular composition of 55 primary (adult) GBM samples by flow cytometry and correlated the tumor immune profile with patient features at diagnosis and outcome. GBM single‐cell suspensions were stained at diagnosis (n = 44) and recurrence following radiotherapy and chemotherapy (n = 11) with a panel of 8‐color monoclonal antibody combinations for the identification and enumeration of (GFAP + CD45 − ) tumor and normal astrocytic cells, infiltrating myeloid cells —i.e. microglial and blood‐derived tumor‐associated macrophages (TAM), M1‐like, and M2‐like TAM, neutrophils. and myeloid‐derived suppressor cells (MDSC)— and tumor‐infiltrating lymphocytes (TIL) —i.e. CD3 + T‐cells and their TCD4 + , TCD8 + , TCD4 − CD8 − , and (CD25 + CD127 lo ) regulatory (T‐regs) subsets, (CD19 + CD20 + ) B‐cells, and (CD16 + ) NK‐cells—. Overall, GBM samples consisted of a major population (mean ± 1SD) of tumor and normal astrocytic cells (73% ± 16%) together with a significant but variable fraction of immune cells (24% ± 18%). Within myeloid cells, TAM predominated (13% ± 12%) including both microglial cells (10% ± 11%) and blood‐derived macrophages (3% ± 5%), in addition to a smaller proportion of neutrophils (5% ± 9%) and MDSC (4% ± 8%). Lymphocytes were less represented and mostly included TCD4 + (0.5% ± 0.7%) and TCD8 + cells (0.6% ± 0.7%), together with lower numbers of TCD4 − CD8 − T‐cells (0.2% ± 0.4%), T‐regs (0.1% ± 0.2%), B‐lymphocytes (0.1% ± 0.2%) and NK‐cells (0.05% ± 0.05%). Overall, three distinct immune profiles were identified: cases with a minor fraction of leucocytes, tumors with a predominance of TAM and neutrophils, and cases with mixed infiltration by TAM, neutrophils, and T‐lymphocytes. Untreated GBM patients with mixed myeloid and lymphoid immune infiltrates showed a significantly shorter patient overall survival versus the other two groups, in the absence of gains of the ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/bpa.12927 |
| الإتاحة: |
https://doi.org/10.1111/bpa.12927Test |
| حقوق: |
http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: |
edsbas.CA20CBB6 |
| قاعدة البيانات: |
BASE |
