دورية أكاديمية
The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice
| العنوان: | The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice |
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| المؤلفون: | M. Kyla Shea, Sarah L. Booth, Stephanie G. Harshman, Donald Smith, Cathy S. Carlson, Lindsey Harper, Alexandra R. Armstrong, Min Fang, M. Leonor Cancela, Márcio Simão, Richard F. Loeser |
| المصدر: | Osteoarthritis and Cartilage Open, Vol 2, Iss 3, Pp 100078- (2020) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Vitamin K, Nutrition, Cartilage, Osteoarthritis, Aging, Rodent model, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Summary: Objective: While a role for vitamin K in maintaining joint tissue homeostasis has been proposed based on the presence of vitamin K dependent proteins in cartilage and bone, it is not clear if low vitamin K intake is causally linked to joint tissue degeneration. To address this gap, we manipulated vitamin K status in aging mice to test its effect on age-related changes in articular cartilage and sub-chondral bone. Methods: Eleven-month old male C57BL6 mice were randomly assigned to a low vitamin K diet containing 120 mcg phylloquinone/kg diet (n = 32) or a control diet containing 1.5 mg phylloquinone/kg diet (n = 30) for 6 months. Knees were evaluated histologically using Safranin O and H&E staining, as well as using micro-CT. Results: Eleven mice in the low vitamin K diet group and three mice in the control group died within the first 100 days of the experiment (p = 0.024). Mice fed the low vitamin K diet had higher Safranin-O scores, indicative of more proteoglycan loss, compared to mice fed the control diet (p ≤ 0.026). The articular cartilage structure scores did not differ between the two groups (p ≥ 0.190). The sub-chondral bone parameters measured using micro CT also did not differ between the two groups (all p ≥ 0.174). Conclusion: Our findings suggest low vitamin K status can promote joint tissue proteoglycan loss in older male mice. Future studies are needed to confirm our findings and obtain a better understanding of the molecular mechanisms underlying the role of vitamin K in joint tissue homeostasis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2665-9131 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2665913120300698Test; https://doaj.org/toc/2665-9131Test; https://doaj.org/article/d10933dfac2842a89f31ef84237a99cdTest |
| DOI: | 10.1016/j.ocarto.2020.100078 |
| الإتاحة: | https://doi.org/10.1016/j.ocarto.2020.100078Test https://doaj.org/article/d10933dfac2842a89f31ef84237a99cdTest |
| رقم الانضمام: | edsbas.C9A1AA36 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 26659131 |
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| DOI: | 10.1016/j.ocarto.2020.100078 |