التفاصيل البيبلوغرافية
| العنوان: |
Meta-analytical biomarker search of EST expression data reveals three differentially expressed candidates |
| المؤلفون: |
Wu, Timothy H, Chu, Lichieh J, Wang, Jian-Chiao, Chen, Ting-Wen, Tien, Yin-Jing, Lin, Wen-Chang, Ng, Wailap V |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2012 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background Researches have been conducted for the identification of differentially expressed genes (DEGs) by generating and mining of cDNA expressed sequence tags (ESTs) for more than a decade. Although the availability of public databases make possible the comprehensive mining of DEGs among the ESTs from multiple tissue types, existing studies usually employed statistics suitable only for two categories. Multi-class test has been developed to enable the finding of tissue specific genes, but subsequent search for cancer genes involves separate two-category test only on the ESTs of the tissue of interest. This constricts the amount of data used. On the other hand, simple pooling of cancer and normal genes from multiple tissue types runs the risk of Simpson's paradox. Here we presented a different approach which searched for multi-cancer DEG candidates by analyzing all pertinent ESTs in all categories and narrowing down the cancer biomarker candidates via integrative analysis with microarray data and selection of secretory and membrane protein genes as well as incorporation of network analysis. Finally, the differential expression patterns of three selected cancer biomarker candidates were confirmed by real-time qPCR analysis. Results Seven hundred and twenty three primary DEG candidates (p-value < 0.05 and lower bound of confidence interval of odds ratio ≧ 1.65) were selected from a curated EST database with the application of Cochran-Mantel-Haenszel statistic (CMH). GeneGO analysis results indicated this set as neoplasm enriched. Cross-examination with microarray data further narrowed the list down to 235 genes, among which 96 had membrane or secretory annotations. After examined the candidates in protein interaction network, public tissue expression databases, and literatures, we selected three genes for further evaluation by real-time qPCR with eight major normal and cancer tissues. The higher-than-normal tissue expression of COL3A1, DLG3, and RNF43 in some of the cancer tissues is in agreement ... |
| نوع الوثيقة: |
conference object |
| اللغة: |
English |
| العلاقة: |
http://www.biomedcentral.com/1471-2164/13/S7/S12Test |
| الإتاحة: |
http://www.biomedcentral.com/1471-2164/13/S7/S12Test |
| حقوق: |
Copyright 2012 Wu et al.; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.C99F71A8 |
| قاعدة البيانات: |
BASE |
