دورية أكاديمية
An essential role for katanin p80 and microtubule severing in male gamete production
| العنوان: | An essential role for katanin p80 and microtubule severing in male gamete production |
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| المؤلفون: | O''Donnell, L, Rhodes, D, Smith, SJ, Merriner, DJ, Borg, C, Whittle, B, O'connor, A, Smith, L, Mcnally, FJ, de Kretser, D, Goodnow, CC, Ormandy, CJ |
| المساهمون: | Frankel, Wayne N |
| المصدر: | urn:ISSN:1553-7390 ; urn:ISSN:1553-7404 ; PLoS Genetics, 8, 5, e1002698 |
| بيانات النشر: | Public Library Science |
| سنة النشر: | 2012 |
| المجموعة: | UNSW Sydney (The University of New South Wales): UNSWorks |
| مصطلحات موضوعية: | Contraception/Reproduction, Genetics, 1.1 Normal biological development and functioning, 1 Underpinning research, Adenosine Triphosphatases, Amino Acid Sequence, Animals, Asthenozoospermia, Gene Expression, Germ Cells, Infertility, Male, Katanin, Meiosis, Mice, Microtubules, Molecular Sequence Data, Mutation, Missense, Oligospermia, Protein Subunits, Sperm Motility, Spermatogenesis, Spermatozoa, Spindle Apparatus, Testis, anzsrc-for: 111201 Cancer Cell Biology, anzsrc-for: 0604 Genetics |
| الوصف: | Katanin is an evolutionarily conserved microtubule-severing complex implicated in multiple aspects of microtubule dynamics. Katanin consists of a p60 severing enzyme and a p80 regulatory subunit. The p80 subunit is thought to regulate complex targeting and severing activity, but its precise role remains elusive. In lower-order species, the katanin complex has been shown to modulate mitotic and female meiotic spindle dynamics and flagella development. The in vivo function of katanin p80 in mammals is unknown. Here we show that katanin p80 is essential for male fertility. Specifically, through an analysis of a mouse loss-of-function allele (the Taily line), we demonstrate that katanin p80, most likely in association with p60, has an essential role in male meiotic spindle assembly and dissolution and the removal of midbody microtubules and, thus, cytokinesis. Katanin p80 also controls the formation, function, and dissolution of a microtubule structure intimately involved in defining sperm head shaping and sperm tail formation, the manchette, and plays a role in the formation of axoneme microtubules. Perturbed katanin p80 function, as evidenced in the Taily mouse, results in male sterility characterized by decreased sperm production, sperm with abnormal head shape, and a virtual absence of progressive motility. Collectively these data demonstrate that katanin p80 serves an essential and evolutionarily conserved role in several aspects of male germ cell development. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/1959.4/unsworks_58955Test; https://unsworks.unsw.edu.au/bitstreams/0126b121-c4b8-4fc8-aa2f-cbfbf154768d/downloadTest; https://doi.org/10.1371/journal.pgen.1002698Test |
| DOI: | 10.1371/journal.pgen.1002698 |
| الإتاحة: | https://doi.org/10.1371/journal.pgen.1002698Test http://hdl.handle.net/1959.4/unsworks_58955Test https://unsworks.unsw.edu.au/bitstreams/0126b121-c4b8-4fc8-aa2f-cbfbf154768d/downloadTest |
| حقوق: | open access ; https://purl.org/coar/access_right/c_abf2Test ; CC-BY-NC-ND ; https://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; free_to_read |
| رقم الانضمام: | edsbas.C910F3C6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pgen.1002698 |
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