دورية أكاديمية
Unravelling the Structural and Molecular Basis Responsible for the Anti-Biofilm Activity of Zosteric Acid.
| العنوان: | Unravelling the Structural and Molecular Basis Responsible for the Anti-Biofilm Activity of Zosteric Acid. |
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| المؤلفون: | Cristina Cattò, Silvia Dell'Orto, Federica Villa, Stefania Villa, Arianna Gelain, Alberto Vitali, Valeria Marzano, Sara Baroni, Fabio Forlani, Francesca Cappitelli |
| المصدر: | PLoS ONE, Vol 10, Iss 7, p e0131519 (2015) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2015 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | The natural compound zosteric acid, or p-(sulfoxy)cinnamic acid (ZA), is proposed as an alternative biocide-free agent suitable for preventive or integrative anti-biofilm approaches. Despite its potential, the lack of information concerning the structural and molecular mechanism of action involved in its anti-biofilm activity has limited efforts to generate more potent anti-biofilm strategies. In this study a 43-member library of small molecules based on ZA scaffold diversity was designed and screened against Escherichia coli to understand the structural requirements necessary for biofilm inhibition at sub-lethal concentrations. Considerations concerning the relationship between structure and anti-biofilm activity revealed that i) the para-sulfoxy ester group is not needed to exploit the anti-biofilm activity of the molecule, it is the cinnamic acid scaffold that is responsible for anti-biofilm performance; ii) the anti-biofilm activity of ZA derivatives depends on the presence of a carboxylate anion and, consequently, on its hydrogen-donating ability; iii) the conjugated aromatic system is instrumental to the anti-biofilm activities of ZA and its analogues. Using a protein pull-down approach, combined with mass spectrometry, the herein-defined active structure of ZA was matrix-immobilized, and was proved to interact with the E. coli NADH:quinone reductase, WrbA, suggesting a possible role of this protein in the biofilm formation process. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | http://europepmc.org/articles/PMC4488431?pdf=renderTest; https://doaj.org/toc/1932-6203Test; https://doaj.org/article/5636b400eea94a3096f5a10b0764bba4Test |
| DOI: | 10.1371/journal.pone.0131519 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0131519Test https://doaj.org/article/5636b400eea94a3096f5a10b0764bba4Test |
| رقم الانضمام: | edsbas.C79EC6BD |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0131519 |