دورية أكاديمية
Single-molecule in vivo imaging of bacterial respiratory complexes indicates delocalized oxidative phosphorylation
| العنوان: | Single-molecule in vivo imaging of bacterial respiratory complexes indicates delocalized oxidative phosphorylation |
|---|---|
| المؤلفون: | Llorente-Garcia, I, Lenn, T, Erhardt, H, Harriman, OL, Liu, L-N, Robson, A, Chiu, S-W, Matthews, S, Willis, NJ, Bray, CD, Lee, S-H, Shin, JY, Bustamante, C, Liphardt, J, Friedrich, T, Mullineaux, CW, Leake, MC |
| المصدر: | Biochimica et Biophysica Acta (BBA) - Bioenergetics , 1837 (6) pp. 811-824. (2014) |
| بيانات النشر: | ELSEVIER |
| سنة النشر: | 2014 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | Cytoplasmic membraneCo-localization analysis, Fluorescence microscopy, Fluorescent protein, Oxidative phosphorylation, Single-molecule biophysics |
| الوصف: | Chemiosmotic energy coupling through oxidative phosphorylation (OXPHOS) is crucial to life, requiring coordinated enzymes whose membrane organization and dynamics are poorly understood. We quantitatively explore localization, stoichiometry, and dynamics of key OXPHOS complexes, functionally fluorescent protein-tagged, in Escherichia coli using low-angle fluorescence and superresolution microscopy, applying single-molecule analysis and novel nanoscale co-localization measurements. Mobile 100–200 nm membrane domains containing tens to hundreds of complexes are indicated. Central to our results is that domains of different functional OXPHOS complexes do not co-localize, but ubiquinone diffusion in the membrane is rapid and long-range, consistent with a mobile carrier shuttling electrons between islands of different complexes. Our results categorically demonstrate that electron transport and proton circuitry in this model bacterium are spatially delocalized over the cell membrane, in stark contrast to mitochondrial bioenergetic supercomplexes. Different organisms use radically different strategies for OXPHOS membrane organization, likely depending on the stability of their environment. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://discovery.ucl.ac.uk/id/eprint/10086642/1/Llorente%20Garcia_1-s2.0-S0005272814000309-main.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10086642Test/ |
| الإتاحة: | https://discovery.ucl.ac.uk/id/eprint/10086642/1/Llorente%20Garcia_1-s2.0-S0005272814000309-main.pdfTest https://discovery.ucl.ac.uk/id/eprint/10086642Test/ |
| حقوق: | open |
| رقم الانضمام: | edsbas.C5297B2C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |