دورية أكاديمية
Detection of Early Esophageal Neoplastic Barrett Lesions with Quantified Fluorescence Molecular Endoscopy Using Cetuximab-800CW
| العنوان: | Detection of Early Esophageal Neoplastic Barrett Lesions with Quantified Fluorescence Molecular Endoscopy Using Cetuximab-800CW |
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| المؤلفون: | Gabriëls, Ruben Y., van Heijst, Lisanne E., Hooghiemstra, Wouter T.R., van der Waaij, Anne M., Kats-Ugurlu, Gursah, Karrenbeld, Arend, Robinson, Dominic J., Tenditnaya, Anna, Ntziachristos, Vasilis, Gorpas, Dimitris, Nagengast, Wouter B. |
| المصدر: | Gabriëls , R Y , van Heijst , L E , Hooghiemstra , W T R , van der Waaij , A M , Kats-Ugurlu , G , Karrenbeld , A , Robinson , D J , Tenditnaya , A , Ntziachristos , V , Gorpas , D & Nagengast , W B 2023 , ' Detection of Early Esophageal Neoplastic Barrett Lesions with Quantified Fluorescence Molecular Endoscopy Using Cetuximab-800CW ' , Journal of Nuclear Medicine , vol. 64 , no. 5 , pp. 803-808 . https://doi.org/10.2967/jnumed.122.264656Test |
| سنة النشر: | 2023 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | Barrett esophagus, cetuximab, epidermal growth factor receptor, esophageal adenocarcinoma, fluorescence molecular imaging |
| الوصف: | Esophageal adenocarcinoma causes 6% of cancer-related deaths worldwide. Near-infrared fluorescence molecular endoscopy (NIR-FME) uses a tracer that targets overexpressed proteins. In this study, we aimed to investigate the feasibility of an epidermal growth factor receptor (EGFR)–targeted tracer, cetuximab-800CW, to improve detection of early-stage esophageal adenocarcinoma. Methods: We validated EGFR expression in 73 esophageal tissue sections. Subsequently, we topically administered cetuximab-800CW and performed high-definition white-light endoscopy (HD-WLE), narrow-band imaging, and NIR-FME in 15 patients with Barrett esophagus (BE). Intrinsic fluorescence values were quantified using multidiameter single-fiber reflectance and single-fiber fluorescence spectroscopy. Back-table imaging, histopathologic examination, and EGFR immunohistochemistry on biopsy samples collected during NIR-FME procedures were performed and compared with in vivo imaging results. Results: Immunohistochemical preanalysis showed high EGFR expression in 67% of dysplastic tissue sections. NIR-FME visualized all 12 HD-WLE–visible lesions and 5 HD-WLE–invisible dysplastic lesions, with increased fluorescence signal in visible dysplastic BE lesions compared with nondysplastic BE as shown by multidiameter single-fiber reflectance/single-fiber fluorescence, reflecting a target-to-background ratio of 1.5. Invisible dysplastic lesions also showed increased fluorescence, with a target-to-background ratio of 1.67. Immunohistochemistry analysis showed EGFR overexpression in 16 of 17 (94%) dysplastic BE lesions, which all showed fluorescence signal. Conclusion: This study has shown that NIR-FME using cetuximab-800CW can improve detection of dysplastic lesions missed by HD-WLE and narrow-band imaging. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://research.rug.nl/en/publications/6e180370-c1ad-45df-a742-84df6fcd3416Test |
| DOI: | 10.2967/jnumed.122.264656 |
| الإتاحة: | https://doi.org/10.2967/jnumed.122.264656Test https://hdl.handle.net/11370/6e180370-c1ad-45df-a742-84df6fcd3416Test https://research.rug.nl/en/publications/6e180370-c1ad-45df-a742-84df6fcd3416Test https://pure.rug.nl/ws/files/760102573/803.full.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C2C5D5F4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2967/jnumed.122.264656 |
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