دورية أكاديمية
β-cell intrinsic dynamics rather than gap junction structure dictates subpopulations in the islet functional network
| العنوان: | β-cell intrinsic dynamics rather than gap junction structure dictates subpopulations in the islet functional network |
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| المؤلفون: | Jennifer K Briggs, Anne Gresch, Isabella Marinelli, JaeAnn M Dwulet, David J Albers, Vira Kravets, Richard KP Benninger |
| المصدر: | eLife, Vol 12 (2023) |
| بيانات النشر: | eLife Sciences Publications Ltd |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | islet, network theory, coordinated oscillations, β-cell oscillations, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Diabetes is caused by the inability of electrically coupled, functionally heterogeneous β-cells within the pancreatic islet to provide adequate insulin secretion. Functional networks have been used to represent synchronized oscillatory [Ca2+] dynamics and to study β-cell subpopulations, which play an important role in driving islet function. The mechanism by which highly synchronized β-cell subpopulations drive islet function is unclear. We used experimental and computational techniques to investigate the relationship between functional networks, structural (gap junction) networks, and intrinsic β-cell dynamics in slow and fast oscillating islets. Highly synchronized subpopulations in the functional network were differentiated by intrinsic dynamics, including metabolic activity and KATP channel conductance, more than structural coupling. Consistent with this, intrinsic dynamics were more predictive of high synchronization in the islet functional network as compared to high levels of structural coupling. Finally, dysfunction of gap junctions, which can occur in diabetes, caused decreases in the efficiency and clustering of the functional network. These results indicate that intrinsic dynamics rather than structure drive connections in the functional network and highly synchronized subpopulations, but gap junctions are still essential for overall network efficiency. These findings deepen our interpretation of functional networks and the formation of functional subpopulations in dynamic tissues such as the islet. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/83147Test; https://doaj.org/toc/2050-084XTest; e83147; https://doaj.org/article/4b9836aacff5411489a467e2968afa6bTest |
| DOI: | 10.7554/eLife.83147 |
| الإتاحة: | https://doi.org/10.7554/eLife.83147Test https://doaj.org/article/4b9836aacff5411489a467e2968afa6bTest |
| رقم الانضمام: | edsbas.C174384 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.83147 |