دورية أكاديمية
A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents
| العنوان: | A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents |
|---|---|
| المؤلفون: | D Premilovac, RJ Gasperini, S Sawyer, A West, Michelle Keske, BV Taylor, L Foa |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, HIGH-FAT DIET, INSULIN-RESISTANCE, RAT, MUSCLE, MECHANISMS, MODEL, OBESITY, type 2 diabetes, metabolic disorder, morbidity, mortality, rodents, 110306 Endocrinology, MD Multidisciplinary, 3202 Clinical sciences |
| الوصف: | Type 2 diabetes is a chronic metabolic disorder that is becoming a leading cause of morbidity and mortality. The prolonged time-course of human type 2 diabetes makes modelling of the disease difficult and additional animal models and methodologies are needed. The goal of this study was to develop and characterise a new method that allows controlled, targeted and sustained induction of discrete stages of type 2 diabetes in rodents. Using adult, male rats, we employed a three-week high fat-diet regimen and confirmed development of obesity-associated glucose intolerance, a key feature of human type 2 diabetes. Next, we utilised osmotic mini-pumps to infuse streptozotocin (STZ; doses ranging 80-200 mg/kg) over the course of 14-days to decrease insulin-producing capacity thus promoting hyperglycemia. Using this new approach, we demonstrate a dose-dependent effect of STZ on circulating glucose and insulin levels as well as glucose tolerance, while retaining a state of obesity. Importantly, we found that insulin secretion in response to a glucose load was present, but reduced in a dose-dependent manner by increasing STZ. In conclusion, we demonstrate a novel method that enables induction of discrete stages of type 2 diabetes in rodents that closely mirrors the different stages of type 2 diabetes in humans. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/10536/DRO/DU:30108714Test; https://figshare.com/articles/journal_contribution/A_new_method_for_targeted_and_sustained_induction_of_type_2_diabetes_in_rodents/20826043Test |
| الإتاحة: | http://hdl.handle.net/10536/DRO/DU:30108714Test https://figshare.com/articles/journal_contribution/A_new_method_for_targeted_and_sustained_induction_of_type_2_diabetes_in_rodents/20826043Test |
| حقوق: | CC BY 4.0 |
| رقم الانضمام: | edsbas.C16F2E1E |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |