دورية أكاديمية
A novel inert crystal delivery medium for serial femtosecond crystallography
| العنوان: | A novel inert crystal delivery medium for serial femtosecond crystallography |
|---|---|
| المؤلفون: | Conrad, Chelsie E., Basu, Shibom, Hunter, Mark S., Koglin, Jason E., Kupitz, Christopher, Nelson, Garrett, Subramanian, Ganesh, White, Thomas, Zhao, Yun, Zook, James, Boutet, Sébastien, Cherezov, Vadim, James, Daniel, Spence, John C. H., Fromme, Raimund, Weierstall, Uwe, Fromme, Petra, Wang, Dingjie, Schaffer, Alexander, Roy-Chowdhury, Shatabdi, Zatsepin, Nadia A., Aquila, Andrew, Coe, Jesse, Gati, Cornelius |
| المصدر: | IUCrJ 2(4), 421 - 430 (2015). doi:10.1107/S2052252515009811 |
| بيانات النشر: | International Union of Crystallography (IUCr) |
| سنة النشر: | 2015 |
| المجموعة: | DESY Publication Database (PUBDB) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/530 |
| جغرافية الموضوع: | DE |
| الوصف: | Serial femtosecond crystallography (SFX) has opened a new era in crystallography by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecondX-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4°C. Using this crystalladen agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 Å resolution using 300 mg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/issn/2052-2525; info:eu-repo/semantics/altIdentifier/wos/WOS:000360065500006; info:eu-repo/semantics/altIdentifier/pmid/pmid:26177184; https://bib-pubdb1.desy.de/record/275879Test; https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2015-04296%22Test |
| الإتاحة: | https://doi.org/10.1107/S2052252515009811Test https://bib-pubdb1.desy.de/record/275879Test https://bib-pubdb1.desy.de/search?p=id:%22PUBDB-2015-04296%22Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C140C10D |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |