دورية أكاديمية
Horizontal gene transfer from human host to HIV-1 reverse transcriptase confers drug resistance and partly compensates for replication deficits.
| العنوان: | Horizontal gene transfer from human host to HIV-1 reverse transcriptase confers drug resistance and partly compensates for replication deficits. |
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| المؤلفون: | Megens, Sarah, VAIRA, Dolorès, De Baets, Greet, Dekeersmaeker, Nathalie, Schrooten, Yoeri, Li, Guangdi, Schymkowitz, Joost, Rousseau, Frederic, Vandamme, Anne-Mieke, Moutschen, Michel, Van Laethem, Kristel |
| المصدر: | Virology, 456-457, 310-8 (2014) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2014 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Chromosomes, Human, Drug Resistance, Viral, Gene Transfer, Horizontal, HIV Infections/virology, HIV Reverse Transcriptase/genetics, HIV-1/drug effects/enzymology/genetics/isolation & purification/physiology, Humans, Models, Molecular, Molecular Sequence Data, Mutagenesis, Insertional, Phylogeny, Protein Conformation, RNA, Viral/genetics, Sequence Analysis, DNA, Sequence Homology, Virus Replication, Antiretroviral therapy, HIV/AIDS, Insertion, Resistance, Reverse transcriptase, Human health sciences, Immunology & infectious disease |
| الوصف: | peer reviewed ; We investigated the origin and the effect of insertion D67D-THGERDLGPA within HIV-1 RT from a patient failing antiviral therapy. The insertion developed within the context of pre-existing NRTI and NNRTI mutations (M41L, L210W, T215Y and N348I). Concurrently, the NRTI mutations T69I and V118I and the NNRTI mutations K103N and Y181C were detected for the first time. High-level drug resistance (fold-changes>/=50) and a good replication capacity (87% of wild-type) were observed, significantly higher than for the previous virus without insertion. The insertion was very similar to a region within human chromosome 17 (31/34 nucleotide identity), and had already been detected independently in a Japanese HIV-1 isolate. These results suggest that a particular sequence within human chromosome 17 is prone to horizontal gene transfer into the HIV-1 RT finger subdomain. This insertion confers selective advantage to HIV-1 by its contribution to multi-drug resistance and restoration of impaired replication capacity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0042-6822 1096-0341 |
| العلاقة: | urn:issn:0042-6822; urn:issn:1096-0341; https://orbi.uliege.be/handle/2268/172537Test; info:hdl:2268/172537; https://orbi.uliege.be/bitstream/2268/172537/1/1-s2.0-S0042682214001056-main.pdfTest; scopus-id:2-s2.0-84899138276; info:pmid:24889250 |
| DOI: | 10.1016/j.virol.2014.03.023 |
| الإتاحة: | https://doi.org/10.1016/j.virol.2014.03.023Test https://orbi.uliege.be/handle/2268/172537Test https://orbi.uliege.be/bitstream/2268/172537/1/1-s2.0-S0042682214001056-main.pdfTest |
| حقوق: | open access ; http://purl.org/coar/access_right/c_abf2Test ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C07A1532 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00426822 10960341 |
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| DOI: | 10.1016/j.virol.2014.03.023 |