دورية أكاديمية
Pheno- and Genotyping of Three Novel Bacteriophage Genera That Target a Wheat Phyllosphere Sphingomonas Genus
| العنوان: | Pheno- and Genotyping of Three Novel Bacteriophage Genera That Target a Wheat Phyllosphere Sphingomonas Genus |
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| المؤلفون: | Riber, Leise, Carstens, Alexander Byth, Dougherty, Peter Erdmann, Roy, Chayan, Willenbücher, Katharina, Hille, Frank, Franz, Charles M.A.P., Hansen, Lars Hestbjerg |
| المصدر: | Riber , L , Carstens , A B , Dougherty , P E , Roy , C , Willenbücher , K , Hille , F , Franz , C M A P & Hansen , L H 2023 , ' Pheno- and Genotyping of Three Novel Bacteriophage Genera That Target a Wheat Phyllosphere Sphingomonas Genus ' , Microorganisms , vol. 11 , no. 7 , 1831 . https://doi.org/10.3390/microorganisms11071831Test |
| سنة النشر: | 2023 |
| المجموعة: | University of Copenhagen: Research / Forskning ved Københavns Universitet |
| مصطلحات موضوعية: | burst size, genomic synteny, intergenomic similarity, latency period, lytic phages, narrow host range, non-prolate Siphovirus, Sphingomonas phages, TEM imaging, wheat flag leaf |
| الوصف: | Bacteriophages are viral agents that infect and replicate within bacterial cells. Despite the increasing importance of phage ecology, environmental phages—particularly those targeting phyllosphere-associated bacteria—remain underexplored, and current genomic databases lack high-quality phage genome sequences linked to specific environmentally important bacteria, such as the ubiquitous sphingomonads. Here, we isolated three novel phages from a Danish wastewater treatment facility. Notably, these phages are among the first discovered to target and regulate a Sphingomonas genus within the wheat phyllosphere microbiome. Two of the phages displayed a non-prolate Siphovirus morphotype and demonstrated a narrow host range when tested against additional Sphingomonas strains. Intergenomic studies revealed limited nucleotide sequence similarity within the isolated phage genomes and to publicly available metagenome data of their closest relatives. Particularly intriguing was the limited homology observed between the DNA polymerase encoding genes of the isolated phages and their closest relatives. Based on these findings, we propose three newly identified genera of viruses: Longusvirus carli, Vexovirus birtae, and Molestusvirus kimi, following the latest ICTV binomial nomenclature for virus species. These results contribute to our current understanding of phage genetic diversity in natural environments and hold promising implications for phage applications in phyllosphere microbiome manipulation strategies. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.3390/microorganisms11071831 |
| الإتاحة: | https://doi.org/10.3390/microorganisms11071831Test https://curis.ku.dk/portal/da/publications/pheno-and-genotyping-of-three-novel-bacteriophage-genera-that-target-a-wheat-phyllosphere-sphingomonas-genusTest(ff307540-0d09-4acf-bca7-becc59f561b9).html https://curis.ku.dk/ws/files/361589055/microorganisms_11_01831.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C01B4439 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/microorganisms11071831 |
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