دورية أكاديمية
Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion
| العنوان: | Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion |
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| المؤلفون: | Martínez-Miguel, Marc, Castellote-Borrell, Miquel, Köber, Mariana, Kyvik, Adriana R., Tomsen-Melero, Judit, Vargas-Nadal, Guillem, Muñoz, Jose, Pulido, Daniel, Cristóbal-Lecina, Edgar, Passemard, Solène, Royo, Miriam, Mas-Torrent, Marta, Veciana, Jaume, Giannotti, Marina I., Guasch, Judith, Ventosa, Nora, Ratera, Imma |
| سنة النشر: | 2022 |
| المجموعة: | Zenodo |
| مصطلحات موضوعية: | nanovesicles, quatsomes, self-assembled monolayers, Arg-Gly-Asp (RGD), cell adhesion, tissue engineering, integrins, surface engineering |
| الوصف: | The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle’s fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/grantAgreement/EC/H2020/801342/; info:eu-repo/grantAgreement/EC/H2020/953110/; info:eu-repo/grantAgreement/EC/H2020/720942/; info:eu-repo/grantAgreement/EC/H2020/101007804/; https://zenodo.org/communities/micro4nanoTest; https://zenodo.org/record/7540227Test; https://doi.org/10.1021/acsami.2c10497Test; oai:zenodo.org:7540227 |
| DOI: | 10.1021/acsami.2c10497 |
| الإتاحة: | https://doi.org/10.1021/acsami.2c10497Test https://zenodo.org/record/7540227Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0/legalcodeTest |
| رقم الانضمام: | edsbas.BF882AA7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acsami.2c10497 |
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