التفاصيل البيبلوغرافية
| العنوان: |
scChIX-seq infers dynamic relationships between histone modifications in single cells |
| المؤلفون: |
Yeung, Jake, Florescu, Maria, Zeller, Peter, de Barbanson, Buys Anton, Wellenstein, Max D., van Oudenaarden, Alexander |
| المساهمون: |
CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Biotechnology, Bioengineering, Biomedical Engineering, Applied Microbiology and Biotechnology, Molecular Medicine |
| الوصف: |
Regulation of chromatin states involves the dynamic interplay between different histone modifications to control gene expression. Recent advances have enabled mapping of histone marks in single cells, but most methods are constrained to profile only one histone mark per cell. Here, we present an integrated experimental and computational framework, scChIX-seq (single-cell chromatin immunocleavage and unmixing sequencing), to map several histone marks in single cells. scChIX-seq multiplexes two histone marks together in single cells, then computationally deconvolves the signal using training data from respective histone mark profiles. This framework learns the cell-type-specific correlation structure between histone marks, and therefore does not require a priori assumptions of their genomic distributions. Using scChIX-seq, we demonstrate multimodal analysis of histone marks in single cells across a range of mark combinations. Modeling dynamics of in vitro macrophage differentiation enables integrated analysis of chromatin velocity. Overall, scChIX-seq unlocks systematic interrogation of the interplay between histone modifications in single cells. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
1087-0156 |
| العلاقة: |
https://dspace.library.uu.nl/handle/1874/448337Test |
| الإتاحة: |
https://dspace.library.uu.nl/handle/1874/448337Test |
| حقوق: |
info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: |
edsbas.BE729A74 |
| قاعدة البيانات: |
BASE |
