دورية أكاديمية
Rhodopseudomonas Palustris Collagen-like recombinant protein purification using an aqueous two-phase system
| العنوان: | Rhodopseudomonas Palustris Collagen-like recombinant protein purification using an aqueous two-phase system |
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| المؤلفون: | Awang, Nursyahidatul Azwa, Amid, Azura, Mohd Arshad, Zatul Iffah |
| بيانات النشر: | International islamic University Malaysia |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | QP Physiology, TP248.13 Biotechnology |
| الوصف: | The potential use of recombinant collagen-like protein (recCLP) extracted from bacteria as disease-free collagen has been studied over the past decade. However, the complexity of the downstream processing generates high demand for an efficient and low-cost purification method. Aqueous two-phase system (ATPS) was adopted as a new approach to the recovery of biomolecules due to its simple, benign, and straightforward process. This study aimed to purify recombinant collagen-like protein from Rhodopseudomonas palustris using ATPS formed by a polymer/salt system. Recombinant collagen-like protein from R. palustris was partitioned in ATPS composed of polyethylene glycol (PEG) and potassium phosphate and several factors that influence the protein partitioning such as volume ratio, system pH, the concentration of polymer and salt were studied. Then, optimization of the selected ATPS conditions (PEG and salt concentration) were performed using response surface methodology (RSM). Results showed that the optimum conditions were found in ATPS with 24.80% (w/w) PEG 2000 and 29.23% (w/w) potassium phosphate with recCLP concentration of 3.23 ± 0.12 mg/mL with purification factor 7.48 ± 0.3. In comparison with the affinity chromatography method, ATPS was found to be low-cost, and time-saving with a higher protein recovery. Hence, this study demonstrated the potential application of ATPS in the recovery of recombinant CLPs for large-scale downstream processing. _______________________________________________________ Potensi penggunaan protein seperti kolagen rekombinan (recCLP) yang diekstrak daripada bakteria sebagai kolagen bebas penyakit telah dikaji sejak sedekad yang lalu. Walau bagaimanapun, kerumitan pemprosesan hiliran menjana permintaan yang tinggi untuk kaedah penulenan yang cekap dan berpatutan. Sistem akueus dua fasa (ATPS) telah diterima pakai sebagai pendekatan baharu dalam pemulihan biomolekul kerana prosesnya yang mudah. Tujuan utama kajian ini adalah untuk menyaring protein seperti kolagen rekombinan daripada ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://irep.iium.edu.my/103100/1/103100_%20Rhodopseudomonas%20Palustris%20Collagen-Like%20Recombinant.pdfTest; http://irep.iium.edu.my/103100/2/IIUM%20Engineering%20Journal%20-%20Journal%20Search%20-%20MyCite.pdfTest; http://irep.iium.edu.my/103100/12/103100_Rhodopseudomonas%20palustris%20COLLAGEN-LIKE%20RECOMBINANT%20PROTEIN_SCOPUS.pdfTest; Awang, Nursyahidatul Azwa and Amid, Azura and Mohd Arshad, Zatul Iffah (2023) Rhodopseudomonas Palustris Collagen-like recombinant protein purification using an aqueous two-phase system. IIUM Engineering Journal, 24 (1). pp. 40-56. ISSN 1511-788X E-ISSN 2289-7860 |
| DOI: | 10.31436/iiumej.v24i1.2468 |
| الإتاحة: | https://doi.org/10.31436/iiumej.v24i1.2468Test http://irep.iium.edu.my/103100Test/ http://irep.iium.edu.my/103100/1/103100_%20Rhodopseudomonas%20Palustris%20Collagen-Like%20Recombinant.pdfTest http://irep.iium.edu.my/103100/2/IIUM%20Engineering%20Journal%20-%20Journal%20Search%20-%20MyCite.pdfTest http://irep.iium.edu.my/103100/12/103100_Rhodopseudomonas%20palustris%20COLLAGEN-LIKE%20RECOMBINANT%20PROTEIN_SCOPUS.pdfTest https://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/2468/904Test |
| رقم الانضمام: | edsbas.BD6F0771 |
| قاعدة البيانات: | BASE |
| DOI: | 10.31436/iiumej.v24i1.2468 |
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