دورية أكاديمية
Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population
| العنوان: | Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population |
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| المؤلفون: | Greenland-Bews, Caitlin, Byrne, Rachel L., Owen, Sophie I., Watkins, Rachel L., Bengey, Daisy, Buist, Kate, Clerkin, Karina, Escadafal, Camille, Finch, Lorna S., Gould, Susan, Giorgi, Emanuele, Hodgkinson, Andy, Mashenko, Larysa, Powell, Darren, Savage, Helen R., Thompson, Caitlin R., Turtle, Lance, Wardale, Jahanara, Wooding, Dominic, Edwards, Thomas, Atienzar, Ana Cubas, Adams, Emily R. |
| المصدر: | BMC Infectious Diseases, Vol 23, Issue 1, e110. |
| بيانات النشر: | BioMed Central |
| سنة النشر: | 2023 |
| المجموعة: | LSTM Online Archive (Liverpool School of Tropical Medicine) |
| مصطلحات موضوعية: | QS 4 General works. Classify here works on regional anatomy, QW 575 Antibodies, QW 806 Vaccination |
| الوصف: | Background: Rapid determination of an individual's antibody status can be beneficial in understanding an individual's immune response to SARS-CoV-2 and for initiation of therapies that are only deemed effective in sero-negative individuals. Antibody lateral flow tests (LFTs) have potential to address this need as a rapid, point of care test. Methods: Here we present a proof-of-concept evaluation of eight LFT brands using sera from 95 vaccinated individuals to determine sensitivity for detecting vaccination generated antibodies. Samples were analysed on eight different brands of antibody LFT and an automated chemiluminescent microparticle immunoassay (CMIA) that identifies anti-spike antibodies which was used as our reference standard. Results: All 95 (100%) participants tested positive for anti-spike antibodies by the chemiluminescent microparticle immunoassay (CMIA) reference standard post-dose two of their SARS-CoV-2 vaccine: BNT162b2 (Pfizer/BioNTech, n = 60), AZD1222 (AstraZeneca, n = 31), mRNA-1273 (Moderna, n = 2) and Undeclared Vaccine Brand (n = 2). Sensitivity increased from dose one to dose two in six out of eight LFTs with three tests achieving 100% sensitivity at dose two in detecting anti-spike antibodies. Conclusions: These tests are demonstrated to be highly sensitive to detect raised antibody levels in vaccinated individuals. RDTs are low cost and rapid alternatives to ELISA based systems. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://archive.lstmed.ac.uk/22054/1/s12879-023-08033-1.pdfTest; Greenland-Bews, Caitlin, Byrne, Rachel L., Owen, Sophie I. orcid:0000-0002-0458-2357 , Watkins, Rachel L., Bengey, Daisy, Buist, Kate, Clerkin, Karina, Escadafal, Camille, Finch, Lorna S., Gould, Susan, Giorgi, Emanuele, Hodgkinson, Andy, Mashenko, Larysa, Powell, Darren, Savage, Helen R., Thompson, Caitlin R., Turtle, Lance, Wardale, Jahanara, Wooding, Dominic, Edwards, Thomas, Atienzar, Ana Cubas and Adams, Emily R. orcid:0000-0002-0816-2835 (2023) 'Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population'. BMC Infectious Diseases, Vol 23, Issue 1, e110. |
| DOI: | 10.1186/s12879-023-08033-1 |
| الإتاحة: | https://doi.org/10.1186/s12879-023-08033-1Test https://archive.lstmed.ac.uk/22054Test/ https://archive.lstmed.ac.uk/22054/1/s12879-023-08033-1.pdfTest |
| حقوق: | cc_by_4 |
| رقم الانضمام: | edsbas.BD04CA52 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12879-023-08033-1 |
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