التفاصيل البيبلوغرافية
| العنوان: |
Functional Characterization of the Plasmodium falciparumand P. berghei Homologues of Macrophage Migration Inhibitory Factor |
| المؤلفون: |
Augustijn, Kevin D., Kleemann, Robert, Thompson, Joanne, Kooistra, Teake, Crawford, Carina E., Reece, Sarah E., Pain, Arnab, Siebum, Arjan H. G., Janse, Chris J., Waters, Andrew P |
| المصدر: |
Infection and Immunity ; volume 75, issue 3, page 1116-1128 ; ISSN 0019-9567 1098-5522 |
| بيانات النشر: |
American Society for Microbiology |
| سنة النشر: |
2007 |
| الوصف: |
Macrophage migration inhibitory factor (MIF) is a mammalian cytokine that participates in innate and adaptive immune responses. Homologues of mammalian MIF have been discovered in parasite species infecting mammalian hosts (nematodes and malaria parasites), which suggests that the parasites express MIF to modulate the host immune response upon infection. Here we report the first biochemical and genetic characterization of a Plasmodium MIF ( P MIF). Like human MIF, histidine-tagged purified recombinant P MIF shows tautomerase and oxidoreductase activities (although the activities are reduced compared to those of histidine-tagged human MIF) and efficiently inhibits AP-1 activity in human embryonic kidney cells. Furthermore, we found that Plasmodium berghei MIF is expressed in both a mammalian host and a mosquito vector and that, in blood stages, it is secreted into the infected erythrocytes and released upon schizont rupture. Mutant P. berghei parasites lacking P MIF were able to complete the entire life cycle and exhibited no significant changes in growth characteristics or virulence features during blood stage infection. However, rodent hosts infected with knockout parasites had significantly higher numbers of circulating reticulocytes. Our results suggest that P MIF is produced by the parasite to influence host immune responses and the course of anemia upon infection. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1128/iai.00902-06 |
| DOI: |
10.1128/IAI.00902-06 |
| الإتاحة: |
https://doi.org/10.1128/iai.00902-06Test |
| حقوق: |
https://journals.asm.org/non-commercial-tdm-licenseTest |
| رقم الانضمام: |
edsbas.BCF2FD21 |
| قاعدة البيانات: |
BASE |
