دورية أكاديمية
Downregulation of hepatic lncRNA Gm19619 improves gluconeogenesis and lipogenesis following vertical sleeve gastrectomy in mice
| العنوان: | Downregulation of hepatic lncRNA Gm19619 improves gluconeogenesis and lipogenesis following vertical sleeve gastrectomy in mice |
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| المؤلفون: | Fang, Zhipeng, Fan, Mingjie, Yuan, Dongqiang, Jin, Lihua, Wang, Yangmeng, Ding, Lili, Xu, Senlin, Tu, Jui, Zhang, Eryun, Wu, Xiwei, Chen, Zhen Bouman, Huang, Wendong |
| المساهمون: | U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences |
| المصدر: | Communications Biology ; volume 6, issue 1 ; ISSN 2399-3642 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Medicine (miscellaneous) |
| الوصف: | Long non-coding RNAs (lncRNAs) are emerging important epigenetic regulators in metabolic processes. Whether they contribute to the metabolic effects of vertical sleeve gastrectomy (VSG), one of the most effective treatments for sustainable weight loss and metabolic improvement, is unknown. Herein, we identify a hepatic lncRNA Gm19619 , which is strongly repressed by VSG but highly up-regulated by diet-induced obesity and overnight-fasting in mice. Forced transcription of Gm19619 in the mouse liver significantly promotes hepatic gluconeogenesis with the elevated expression of G6pc and Pck1. In contrast, AAV-CasRx mediated knockdown of Gm19619 in high-fat diet-fed mice significantly improves hepatic glucose and lipid metabolism. Mechanistically, Gm19619 is enriched along genomic regions encoding leptin receptor (Lepr) and transcription factor Foxo1, as revealed in chromatin isolation by RNA purification (ChIRP) assay and is confirmed to modulate their transcription in the mouse liver. In conclusion, Gm19619 may enhance gluconeogenesis and lipid accumulation in the liver. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s42003-023-04483-0 |
| الإتاحة: | https://doi.org/10.1038/s42003-023-04483-0Test https://www.nature.com/articles/s42003-023-04483-0.pdfTest https://www.nature.com/articles/s42003-023-04483-0Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.BCA0E000 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s42003-023-04483-0 |
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