دورية أكاديمية
Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
| العنوان: | Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii |
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| المؤلفون: | Crozet, Pierre, Navarro, Francisco, Willmund, Felix, Mehrshahi, Payam, Bakowski, Kamil, Lauersen, Kyle, Pérez-Pérez, Maria-Esther, Auroy, Pascaline, Gorchs Rovira, Aleix, Sauret-Gueto, Susana, Niemeyer, Justus, Spaniol, Benjamin, Theis, Jasmine, Trösch, Raphael, Westrich, Lisa-Desiree, Vavitsas, Konstantinos, Baier, Thomas, Hübner, Wolfgang, de Carpentier, Felix, Cassarini, Mathieu, Danon, Antoine, Henri, Julien, Marchand, Christophe, de Mia, Marcello, Sarkissian, Kevin, Baulcombe, David, Peltier, G., Crespo, José-Luis, Kruse, Olaf, Jensen, Poul-Erik, Schroda, Michael, Smith, Alison, Lemaire, Stéphane |
| المساهمون: | Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Cambridge UK (CAM), Technische Universität Kaiserslautern (TU Kaiserslautern), University of Copenhagen = Københavns Universitet (UCPH), Universität Bielefeld = Bielefeld University, Universidad de Sevilla = University of Seville, Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ANR-17-CE05-0008,MIDWAY,Contrôle des Interfaces Electrochimiques pour de meilleurs electrocatalyseurs pour l'oxydation de l'eau(2017) |
| المصدر: | ISSN: 2161-5063 ; ACS Synthetic Biology ; https://hal.sorbonne-universite.fr/hal-01909673Test ; ACS Synthetic Biology, 2018, 7 (9), pp.2074-2086. ⟨10.1021/acssynbio.8b00251⟩. |
| بيانات النشر: | HAL CCSD American Chemical Society |
| سنة النشر: | 2018 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | modular cloning, Chlamydomonas reinhardtii, synthetic biology, algal biotechnology, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics |
| الوصف: | International audience ; Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01909673; https://hal.sorbonne-universite.fr/hal-01909673Test; https://hal.sorbonne-universite.fr/hal-01909673/documentTest; https://hal.sorbonne-universite.fr/hal-01909673/file/Crozet_%26_Lemaire-preprint-2018-ACS_SynBio.pdfTest |
| DOI: | 10.1021/acssynbio.8b00251 |
| الإتاحة: | https://doi.org/10.1021/acssynbio.8b00251Test https://hal.sorbonne-universite.fr/hal-01909673Test https://hal.sorbonne-universite.fr/hal-01909673/documentTest https://hal.sorbonne-universite.fr/hal-01909673/file/Crozet_%26_Lemaire-preprint-2018-ACS_SynBio.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.BC95282A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acssynbio.8b00251 |
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