دورية أكاديمية
SOLEIL shining on the solution-state structure of biomacromolecules by synchrotron X-ray footprinting at the metrology beamline
| العنوان: | SOLEIL shining on the solution-state structure of biomacromolecules by synchrotron X-ray footprinting at the metrology beamline |
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| المؤلفون: | Baud, Anna, Aymé, Laure, Gonnet, Florence, Salard, Isabelle, Gohon, Yann, Jolivet, Pascale, Brodolin, Konstantin, da Silva, P., Giuliani, Alexandre, Sclavi, B., Chardot, Thierry, Mercere, P., Roblin, Pierre, Daniel, Régis |
| المساهمون: | Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Saclay (COmUE), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre d’études d’Agents Pathogènes et Biotechologies pour la Santé (CPBS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Département Caractérisation et Elaboration des Produits Issus de l'Agriculture (CEPIA), Institut National de la Recherche Agronomique (INRA), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS), SOLEIL 20120893, 20130817, 20140760, INRA CEPIA department: OlSoleilMiox project |
| المصدر: | ISSN: 0909-0495. |
| بيانات النشر: | HAL CCSD International Union of Crystallography |
| سنة النشر: | 2017 |
| المجموعة: | AgroParisTech: HAL (Institut des sciences et industries du vivant et de l'environnement) |
| مصطلحات موضوعية: | factor H, C3b, mass spectrometry, X-ray footprinting, structural proteomics, S3 oleosin, [SDV]Life Sciences [q-bio], [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] |
| الوصف: | Synchrotron X-ray footprinting complements the techniques commonly used to define the structure of molecules such as crystallography, small-angle X-ray scattering and nuclear magnetic resonance. It is remarkably useful in probing the structure and interactions of proteins with lipids, nucleic acids or with other proteins in solution, often better reflecting the in vivo state dynamics. To date, most X-ray footprinting studies have been carried out at the National Synchrotron Light Source, USA, and at the European Synchrotron Radiation Facility in Grenoble, France. This work presents X-ray footprinting of biomolecules performed for the first time at the X-ray Metrology beamline at the SOLEIL synchrotron radiation source. The installation at this beamline of a stopped-flow apparatus for sample delivery, an irradiation capillary and an automatic sample collector enabled the X-ray footprinting study of the structure of the soluble protein factor H (FH) from the human complement system as well as of the lipid-associated hydrophobic protein S3 oleosin from plant seed. Mass spectrometry analysis showed that the structural integrity of both proteins was not affected by the short exposition to the oxygen radicals produced during the irradiation. Irradiated molecules were subsequently analysed using high-resolution mass spectrometry to identify and locate oxidized amino acids. Moreover, the analyses of FH in its free state and in complex with complement C3b protein have allowed us to create a map of reactive solvent-exposed residues on the surface of FH and to observe the changes in oxidation of FH residues upon C3b binding. Studies of the solvent accessibility of the S3 oleosin show that X-ray footprinting offers also a unique approach to studying the structure of proteins embedded within membranes or lipid bodies. All the biomolecular applications reported herein demonstrate that the Metrology beamline at SOLEIL can be successfully used for synchrotron X-ray footprinting of biomolecules. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28452748; hal-01608337; https://hal.science/hal-01608337Test; https://hal.science/hal-01608337/documentTest; https://hal.science/hal-01608337/file/2017_Baud_J.%20Synchrotron%20Rad_1.pdfTest; PRODINRA: 398098; PUBMED: 28452748; WOS: 000400385400004 |
| DOI: | 10.1107/S1600577517002478 |
| الإتاحة: | https://doi.org/10.1107/S1600577517002478Test https://hal.science/hal-01608337Test https://hal.science/hal-01608337/documentTest https://hal.science/hal-01608337/file/2017_Baud_J.%20Synchrotron%20Rad_1.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.BC75C67B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1107/S1600577517002478 |
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