التفاصيل البيبلوغرافية
| العنوان: |
Improved transplantation efficiency hepatocytes expressing Ser45‐mutated‐β‐catenin |
| المؤلفون: |
Nejak‐Bowen, Kari Nichole, Monga, Satdarshan |
| المصدر: |
The FASEB Journal ; volume 24, issue S1 ; ISSN 0892-6638 1530-6860 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2010 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Because healthy, non‐diseased donor livers for orthotopic liver transplantation are scarce, the development of alternative sources of functioning hepatocytes as a bridge to transplantation is critical. However, the availability of viable hepatocytes in numbers large enough for use in cell therapy is currently limited. Hepatocytes overexpressing a mutated form of β‐catenin may have a homing, growth, and regenerative advantage over wild‐type (WT) cells, thus decreasing the numbers of cells needed for transplantation. Our laboratory has previously shown that transgenic (TG) hepatocytes overexpressing Ser45‐mutated‐β‐catenin proliferate faster in culture than WT hepatocytes and show increased viability after 120 hours. However, the replicative capacity of these TG hepatocytes is unchanged from that of WT, as they senesce at approximately the same number of passages as WT hepatocytes in long‐term culture. Addtionally, expression of telomerase is equivalent in both WT and TG livers, indicating that TG hepatocytes containing mutated β‐catenin do not proliferate indefinitely. Preliminary results suggest that transplanted (Tx) WT hepatocytes can contribute to the livers of hepatectomized (PHx) β‐catenin KO livers (a model of delayed regeneration), but do not contribute to the livers of WT mice. Furthermore, the reduction in AST and ALT seen in PHx/Tx mice at D30 suggests that these transplanted hepatocytes may help to ameliorate damage and improve function. Currently ongoing cell transplantation experiments in which hepatocytes overexpressing TG mutated β‐catenin are transplanted into KO animals will elucidate the role of β‐catenin in repopulation of damaged livers. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1096/fasebj.24.1_supplement.749.2 |
| الإتاحة: |
https://doi.org/10.1096/fasebj.24.1_supplement.749.2Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.BC7446C2 |
| قاعدة البيانات: |
BASE |
