دورية أكاديمية
Analysis of interleukin-4 and interleukin-10 and their association with the lymphocytic infiltrate in the small intestine of patients with coeliac disease.
| العنوان: | Analysis of interleukin-4 and interleukin-10 and their association with the lymphocytic infiltrate in the small intestine of patients with coeliac disease. |
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| المؤلفون: | Beckett, C G, Dell'Olio, D, Kontakou, M, Przemioslo, R T, Rosen-Bronson, S, Ciclitira, P J |
| بيانات النشر: | BMJ Publishing Group Ltd |
| سنة النشر: | 1996 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Research Article |
| الوصف: | BACKGROUND: Concentrations of pro-inflammatory cytokines are raised in the small intestine of patients with coeliac disease after ingestion of gluten but there are equivalent data on interleukin-4 (IL-4) and interleukin-10 (IL-10) producing cells. These cytokines are known to exert important regulatory effects on pro-inflammatory cytokine production from lymphocytes and macrophages. AIMS: To investigate whether there is a primary deficiency of IL-4 and IL-10 producing cells and their site of production in the small intestine of patients with coeliac disease in relation to the changes in inflammatory cell infiltrate. PATIENTS: Jejunal biopsy specimens from patients with coeliac disease (11 untreated, 10 treated) and nine disease controls were studied. METHODS: Immunohistochemical staining of sections for IL-4 and IL-10 cytokines and the cell phenotypic markers CD3 (T lymphocytes) and CD45 (total inflammatory cell infiltrate) was carried out using monoclonal antibodies. Expression of IL-4 and IL-10 messenger RNA was detected by in situ hybridisation with oligonucleotide probe cocktails for each cytokine. RESULTS: IL-4 and IL-10 mRNA and protein were detected in the lamina propria of treated and untreated coeliac patients and disease controls but not in the epithelium. A significant increase in the number of CD45 (p < 0.005) and CD3 (p < 0.05) positive cells was found in the lamina propria of patients with untreated coeliac disease compared with treated coeliac patients and disease controls but there were no differences in IL-4 or IL-10 between these groups with either method. CONCLUSIONS: There is no primary deficiency of IL-4 and IL-10 producing cells in the small intestine of patients with coeliac disease. Detectable concentrations of IL-4 and IL-10 were found in control patients which suggests that these cytokines are involved in normal mucosal immunoregulation. The increased number of T lymphocytes but not IL-4 or IL-10 producing cells in the lamina propria of patients with untreated than in those with ... |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://gut.bmj.com/cgi/content/short/39/6/818Test; http://dx.doi.org/10.1136/gut.39.6.818Test |
| DOI: | 10.1136/gut.39.6.818 |
| الإتاحة: | https://doi.org/10.1136/gut.39.6.818Test http://gut.bmj.com/cgi/content/short/39/6/818Test |
| حقوق: | Copyright (C) 1996, BMJ Publishing Group |
| رقم الانضمام: | edsbas.BC404AAA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/gut.39.6.818 |
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