دورية أكاديمية

Dynamic profiling of the immune tumor microenvironment in locally advanced gastric cancer treated with perioperative chemotherapy

التفاصيل البيبلوغرافية
العنوان: Dynamic profiling of the immune tumor microenvironment in locally advanced gastric cancer treated with perioperative chemotherapy
المؤلفون: Pecora, Irene, Ugolini, Clara, Giannini, Riccardo, Giordano, Mirella, Vivaldi, Caterina, Lencioni, Monica, Santi, Stefano, Massa, Valentina, Pallabazzer, Giovanni, Catanese, Silvia, Salani, Francesca, Belluomini, Mario Antonio, Vasile, Enrico, Cremolini, Chiara, Fontanini, Gabriella, Masi, Gianluca, Fornaro, Lorenzo
المساهمون: Pecora, Irene, Ugolini, Clara, Giannini, Riccardo, Giordano, Mirella, Vivaldi, Caterina, Lencioni, Monica, Santi, Stefano, Massa, Valentina, Pallabazzer, Giovanni, Catanese, Silvia, Salani, Francesca, Belluomini, Mario Antonio, Vasile, Enrico, Cremolini, Chiara, Fontanini, Gabriella, Masi, Gianluca, Fornaro, Lorenzo
سنة النشر: 2023
المجموعة: ARPI - Archivio della Ricerca dell'Università di Pisa
مصطلحات موضوعية: Gastric cancer, NanoString, Immune profiling, Tumor microenvironment, 5-Fluorouracil/leucovorin, oxaliplatin, docetaxel
الوصف: Introduction: In locally advanced gastric cancer (GC), FLOT represents the standard perioperative regimen and combination with immunotherapy is under investigation. However, the role of immune tumor microenvironment (TME) is poorly recognized in this setting. We aimed to study TME characteristics and dynamics during FLOT. Methods: Paired biopsy (PRE) and surgical (POST) samples of 25 patients treated with FLOT were prospectively analyzed. After collection of clinic-pathological data, NanoString analyses were performed. The primary objective of the study was to assess the changes induced by chemotherapy in POST compared to PRE samples. Results: The unsupervised hierarchical method analysis clearly distinguished PRE and POST samples, even though some cases showed high immune gene expression at baseline. When POST samples were compared with PRE, a differential expression in hyper-expressed gene sets related to cytotoxicity, T-cell functions, complement system, tumor necrosis factor-superfamily, cell cycle and regulation was recognized. Downstaging of the primary tumor (T-regression, measured by pathologic compared to clinical T stage) was the covariate most frequently associated with these changes. Using the immune cell profiling, cases with T-regression reported a significant increase of T, CD8+ T and B-cells and a decrease in mast cells, while non-responders demonstrated an increase of T, B, cytotoxic and mast cells. Conclusion: Our analysis shows that FLOT significantly influences immune TME of GC. While relevant modifications preferentially occur in tumors showing primary tumor regression, response to treatment seems to be associated with a specific immune profile.
نوع الوثيقة: article in journal/newspaper
اللغة: English
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37307799; info:eu-repo/semantics/altIdentifier/wos/WOS:001010250500001; journal:ONCOLOGY; https://hdl.handle.net/11568/1184412Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85167842098; https://karger.com/ocl/article-abstract/101/7/435/845116/Dynamic-Profiling-of-the-Immune-Tumor?redirectedFrom=fulltextTest
DOI: 10.1159/000530853
الإتاحة: https://doi.org/10.1159/000530853Test
https://hdl.handle.net/11568/1184412Test
https://karger.com/ocl/article-abstract/101/7/435/845116/Dynamic-Profiling-of-the-Immune-Tumor?redirectedFrom=fulltextTest
رقم الانضمام: edsbas.BB04C10A
قاعدة البيانات: BASE