التفاصيل البيبلوغرافية
| العنوان: |
The endometrial transcriptome transition preceding receptivity to embryo implantation in mice |
| المؤلفون: |
Chan, Hon Yeung, Tran, Ha M., Breen, James, Schjenken, John E., Robertson, Sarah A. |
| المساهمون: |
National Health and Medical Research Council, Australian Research Council |
| المصدر: |
BMC Genomics ; volume 24, issue 1 ; ISSN 1471-2164 |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Genetics, Biotechnology |
| الوصف: |
Background Receptivity of the uterus is essential for embryo implantation and progression of mammalian pregnancy. Acquisition of receptivity involves major molecular and cellular changes in the endometrial lining of the uterus from a non-receptive state at ovulation, to a receptive state several days later. The precise molecular mechanisms underlying this transition and their upstream regulators remain to be fully characterized. Here, we aimed to generate a comprehensive profile of the endometrial transcriptome in the peri-ovulatory and peri-implantation states, to define the genes and gene pathways that are different between these states, and to identify new candidate upstream regulators of this transition, in the mouse. Results High throughput RNA-sequencing was utilized to identify genes and pathways expressed in the endometrium of female C57Bl/6 mice at estrus and on day 3.5 post-coitum (pc) after mating with BALB/c males (n = 3–4 biological replicates). Compared to the endometrium at estrus, 388 genes were considered differentially expressed in the endometrium on day 3.5 post-coitum. The transcriptional changes indicated substantial modulation of uterine immune and vascular systems during the pre-implantation phase, with the functional terms Angiogenesis, Chemotaxis, and Lymphangiogenesis predominating. Ingenuity Pathway Analysis software predicted the activation of several upstream regulators previously shown to be involved in the transition to receptivity including various cytokines, ovarian steroid hormones, prostaglandin E2, and vascular endothelial growth factor A. Our analysis also revealed four candidate upstream regulators that have not previously been implicated in the acquisition of uterine receptivity, with growth differentiation factor 2, lysine acetyltransferase 6 A, and N-6 adenine-specific DNA methyltransferase 1 predicted to be activated, and peptidylprolyl isomerase F predicted to be inhibited. Conclusions This study confirms that the transcriptome of a receptive uterus is vastly ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1186/s12864-023-09698-3 |
| DOI: |
10.1186/s12864-023-09698-3.pdf |
| DOI: |
10.1186/s12864-023-09698-3/fulltext.html |
| الإتاحة: |
https://doi.org/10.1186/s12864-023-09698-3Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: |
edsbas.B9B4B057 |
| قاعدة البيانات: |
BASE |
