التفاصيل البيبلوغرافية
| العنوان: |
Amphiregulin improves ventricular remodeling after myocardial infarction by modulating autophagy and apoptosis |
| المؤلفون: |
Li, Nana, Xia, Ni, He, Junyi, Liu, Meilin, Gu, Muyang, Lu, Yuzhi, Yang, Haoyi, Hao, Zhiheng, Zha, Lingfeng, Wang, Xuhong, Wang, Weimin, Hu, Desheng, Hu, Jiong, Cheng, Xiang |
| المساهمون: |
National Natural Science Foundation of China |
| المصدر: |
The FASEB Journal ; volume 38, issue 4 ; ISSN 0892-6638 1530-6860 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2024 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Myocardial infarction (MI) is defined as sudden ischemic death of myocardial tissue. Amphiregulin (Areg) regulates cell survival and is crucial for the healing of tissues after damage. However, the functions and mechanisms of Areg after MI remain unclear. Here, we aimed to investigate Areg's impact on myocardial remodeling. Mice model of MI was constructed and Areg −/− mice were used. Expression of Areg was analyzed using western blotting, RT–qPCR, flow cytometry, and immunofluorescence staining. Echocardiographic analysis, Masson's trichrome, and triphenyltetrazolium chloride staining were used to assess cardiac function and structure. RNA sequencing was used for unbiased analysis. Apoptosis and autophagy were determined by western blotting, TUNEL staining, electron microscopy, and mRFP‐GFP‐LC3 lentivirus. Lysosomal acidity was determined by Lysotracker staining. Areg was elevated in the infarct border zone after MI. It was mostly secreted by macrophages. Areg deficiency aggravated adverse ventricular remodeling, as reflected by worsening cardiac function, a lower survival rate, increased scar size, and interstitial fibrosis. RNA sequencing analyses showed that Areg related to the epidermal growth factor receptor (EGFR), phosphoinositide 3‐kinase/protein kinase B (PI3K‐Akt), mammalian target of rapamycin (mTOR) signaling pathways, V‐ATPase and lysosome pathways. Mechanistically, Areg exerts beneficial effects via increasing lysosomal acidity to promote autophagosome clearance, and activating the EGFR/PI3K/Akt/mTOR signaling pathway, subsequently inhibiting excessive autophagosome formation and apoptosis in cardiomyocytes. This study provides a novel evidence for the role of Areg in inhibiting ventricular remodeling after MI by regulating autophagy and apoptosis and identifies Areg as a potential therapeutic target in ventricular remodeling after MI. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1096/fj.202302385r |
| الإتاحة: |
https://doi.org/10.1096/fj.202302385rTest |
| حقوق: |
http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: |
edsbas.B96D5412 |
| قاعدة البيانات: |
BASE |
