دورية أكاديمية
Mechanism of S100b release from rat cortical slices determined under basal and stimulated conditions
| العنوان: | Mechanism of S100b release from rat cortical slices determined under basal and stimulated conditions |
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| المساهمون: | Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Farmakoloji Anabilim Dalı., Gürsoy, Murat, Büyükuysal, Rıfat Levent, AAH-1657-2021, 57197640824, 6602686612 |
| بيانات النشر: | Springer/Plenum Publishers |
| سنة النشر: | 2010 |
| المجموعة: | Açık Erişim@BUU (Bursa Uludağ Üniversitesi) |
| مصطلحات موضوعية: | S100B release, Oxygen-glucose deprivation (OGD), Re-oxygenation, Protein-kinase-C, Delayed neuronal injury, In-vitro trauma, Cerebral-ischemia, Brain-slices, Hippocampal-neurons, Damage, Secretion, Glutamate, Toxicity, Biochemistry & molecular biology, Neurosciences & neurology, Rattus, Animals, Calcium channel blockers, Cerebral cortex, Enzyme activation, Enzyme inhibitors, Female, Glucose, Glutamic acid, Hypoxia, brain, Ketoglutaric acids, L-lactate dehydrogenase, Male, Nerve growth factors |
| الوصف: | Incubation of rat cortical slices in a medium that was not containing oxygen and glucose (oxygen-glucose deprivation, OGD) caused a 200% increase in the release of S100B. However, when slices were transferred to a medium containing oxygen and glucose (reoxygenation conditions, or REO), S100B release reached 500% of its control value. Neither inhibition of nitric oxide (NO) synthase by L-NAME nor addition of the NO donors sodium nitroprussid (SNP) or hydroxylamine (HA) to the medium altered basal S100B release. Similarly, the presence of SNP, HA or NO precursor l-arginine in the medium, or inhibition of NO synthase by L-NAME also failed to alter OGD- and REO-induced S100B outputs. Moreover, individual inhibition of PKC, PLA(2) or PLC all failed to attenuate the S100B release determined under control condition or enhanced by either OGD or REO. Blockade of calcium channels with verapamil, chelating the Ca+2 ions with BAPTA or blockade of sodium channels with tetrodotoxin (TTX) did not alter OGD- and REO-induced S100B release. In contrast to the pharmacologic manipulations mentioned above, glutamate and alpha-ketoglutarate added at high concentrations to the medium prevented both OGD- and REO-induced S100B outputs. These results indicate that neither NO nor the activation of PKC, PLA(2) or PLC seem to be involved in basal or OGD- and REO-induced S100B outputs. Additionally, calcium and sodium currents that are sensitive to verapamil and TTX, respectively, are unlikely to contribute to the enhanced S100B release observed under these conditions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0364-3190 1573-6903 19823932 |
| العلاقة: | Makale - Uluslararası Hakemli Dergi; 2006/49; Neurochemical Research; Gürsoy, M. ve Büyükuysal, R. L. (2010). "Mechanism of S100b release from rat cortical slices determined under basal and stimulated conditions". Neurochemical Research, 35(3), 429-436.; https://doi.org/10.1007/s11064-009-0075-9Test; https://link.springer.com/article/10.1007/s11064-009-0075-9Test; http://hdl.handle.net/11452/25400Test; 000274403600010; 2-s2.0-76849087262; 429; 436; 35 |
| DOI: | 10.1007/s11064-009-0075-9 |
| الإتاحة: | https://doi.org/10.1007/s11064-009-0075-9Test http://hdl.handle.net/11452/25400Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.B8912589 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 03643190 15736903 19823932 |
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| DOI: | 10.1007/s11064-009-0075-9 |